202114-53-2 Usage
Uses
Used in Peptide and Protein Synthesis:
FMOC-LEU-OH (C1-13C) is used as a building block in the synthesis of peptides and proteins. Its role is crucial for the stepwise assembly of peptide chains during solid-phase synthesis, where the FMOC group protects the N-terminus from unwanted reactions until it is strategically removed.
Used in Research of Protein Structure and Dynamics:
FMOC-LEU-OH (C1-13C) is utilized as a labeled compound for studying the structural and dynamic properties of proteins. The 13C isotope allows researchers to track the specific carbon atom within the protein structure using techniques such as nuclear magnetic resonance (NMR) spectroscopy, providing insights into protein folding, stability, and interactions.
Used in Metabolic Studies:
In metabolic research, FMOC-LEU-OH (C1-13C) serves as a tracer molecule. The 13C label aids in tracking the metabolic fate of the amino acid derivative within biological systems, which is valuable for understanding metabolic pathways and the effects of interventions or drug candidates on these pathways.
Used in Pharmaceutical Development:
FMOC-LEU-OH (C1-13C) is employed in the development of pharmaceuticals, particularly in the design and synthesis of peptide-based drugs. Its use ensures that the synthesized peptides have the desired properties, and the 13C label can be instrumental in studying the drug's pharmacokinetics and pharmacodynamics.
Used in Analytical Chemistry:
FMOC-LEU-OH (C1-13C) is used as an internal standard or a reference compound in analytical chemistry. The 13C labeling provides a means to accurately quantify and compare the behavior of other compounds in complex mixtures, enhancing the precision and reliability of analytical methods.
Check Digit Verification of cas no
The CAS Registry Mumber 202114-53-2 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 2,0,2,1,1 and 4 respectively; the second part has 2 digits, 5 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 202114-53:
(8*2)+(7*0)+(6*2)+(5*1)+(4*1)+(3*4)+(2*5)+(1*3)=62
62 % 10 = 2
So 202114-53-2 is a valid CAS Registry Number.
202114-53-2Relevant academic research and scientific papers
Evaluation of dihedral angles of peptides using IR bands of two successive isotope labeled residues
Okabe, Hitomi,Miyata, Daisuke,Nakabayashi, Takakazu,Hiramatsu, Hirotsugu
, p. 80 - 86 (2019/01/28)
The infrared (IR) absorption bands due to peptide bonds (amide bands) have long been used to determine the secondary structure of a peptide and to analyze intra- and intermolecular interactions between amides. In the present study, the dihedral angles of a residue in peptides have also been evaluated using the amide I IR band of two successive residues with isotope labeling. The two successive residues labeled with the13C and18O isotopes give the doublet amide I IR band and the intensity ratio (Rint) and the difference in peak position (|ν) of the doublet band were analyzed using GF matrix and ab initio molecular orbital calculations. We obtained the two-dimensional calculation maps of Rint and |ν against the two dihedral angles. The crossing point of the curves of Rint and |ν is the two dihedral angles of the measured residue. The evaluated dihedral angles of the simple peptides are compared with the reported values. We discuss the limitation and the application of the present method to biopolymers from the obtained results.
A simple and economical method for the production of13C, 18O-labeled fmoc-amino acids with high levels of enrichment: Applications to isotope-edited IR studies of proteins
Marecek, James,Song, BenBen,Brewery, Scott,Belyea, Jenifer,Dyer, R. Brian,Raleigh, Daniel P.
, p. 4935 - 4938 (2008/03/28)
Isotope-edited IR of proteins has generated considerable interest. Double labeling with 13C and 18O with high levels of isotopic enrichment is required for residue-specific resolution. Current methods for the preparation of doubly la
