205379-08-4

Basic information

• Product Name: N-(5-AMINO-1-CARBOXYPENTYL)IMINODIACETIC ACID, TRI-T-BUTYL ESTER
• Synonyms: N-(5-AMINO-1-CARBOXYPENTYL)IMINODIACETIC ACID, TRI-T-BUTYL ESTER;N2,N2-Bis[2-(1,1-diMethylethoxy)-2-oxoethyl]-L-lysine 1,1-DiMethylethyl Ester;L-Lysine, N2,N2-bis[2-(1,1-diMethylethoxy)-2-oxoethyl]-, 1,1-diMethylethyl ester
• CAS NO: 205379-08-4
• Molecular Formula: C₂₂H₄₂N₂O₆
• Molecular Weight: 374.47
• Product Categories: Chelating Agents, Ligands;Amino Acids & Derivatives;Chelating Agents & Ligands
• Mol File: 205379-08-4.mol
• Article Data: 18

Chemical Properties

• Boiling Point: 484.68°C at 760 mmHg
• Flash Point: 246.926°C
• Appearance: /
• Density: 1.041g/cm³
• Vapor Pressure: 0mmHg at 25°C
• Refractive Index: 1.475
• Storage Temp.: -20°C Freezer
• Solubility: Chloroform (Slightly), Methanol (Slightly)
• Stability: Moisture Sensitive
• CAS DataBase Reference: N-(5-AMINO-1-CARBOXYPENTYL)IMINODIACETIC ACID, TRI-T-BUTYL ESTER(CAS DataBase Reference)
• NIST Chemistry Reference: N-(5-AMINO-1-CARBOXYPENTYL)IMINODIACETIC ACID, TRI-T-BUTYL ESTER(205379-08-4)
• EPA Substance Registry System: N-(5-AMINO-1-CARBOXYPENTYL)IMINODIACETIC ACID, TRI-T-BUTYL ESTER(205379-08-4)

Safety Data

• Hazardous Substances Data: 205379-08-4(Hazardous Substances Data)

Usage

Chemical Properties

Yellow Oil

Uses

N-(5-AMino-1-carboxypentyl)iMinodiacetic Acid Tri-t-butyl Ester is a nitrilotriacetic acid derivative used as a metal chelating adsorbent for metal ion affinity chromatography. This method can be used for identification and rapid one-step purification of gene produc ts expressed as fusion proteins with an oligo-histidine tag. The oligo-histidine tag serves as a high affinity binding sequence for the purification of fusion proteins via a metal chelating absorbent such as N-(5-Amino-1-carboxypentyl)iminodiacetic acid.

InChI:InChI=1/C22H42N2O6/c1-20(2,3)28-17(25)14-24(15-18(26)29-21(4,5)6)16(12-10-11-13-23)19(27)30-22(7,8)9/h16H,10-15,23H2,1-9H3/t16-/m1/s1

Upstream product

• 205379-07-3 N^α,N^α-bis[(tert-butyloxycarbonyl)methyl]-N^ε-benzyloxycarbonyl-L-lysine tert-butyl ester 
• 135727-85-4 N²-[(1,1-dimethylethoxy)carbonyl]-N⁶-[(phenylmethoxy)carbonyl]-L-lysine 1,1-dimethylethyl ester 
• 2389-45-9 Boc-Lys(Z)-OH 
• 1155-64-2 N₆-[(benzyloxy)carbonyl]-L-lysine 
• 5978-22-3 N(ε)-benzoyloxycarbonyl-L-lysine tert-butyl ester hydrochloride 
• 63628-63-7 Nε-benzyloxycarbonyl-L-lysine tert-butyl ester 
• 5292-43-3 bromoacetic acid tert-butyl ester 

Downstream Products

• 1241912-74-2 C₃₁H₂₉N₃O₁₁S 
• 113231-05-3 N,N-bis(carboxymethyl)-L-lysine 
• 862778-50-5 TO-bis NTA 

Relevant articles and documents

Grid coatings for capture of proteins and other compounds

Grids comprising a coating modified with one or more capture agents and a deactivating agent are disclosed. Methods of using such grids in connection with suitable microscopy techniques, such as for determining the structure of target compounds including proteins, are also disclosed.

Optimized reaction pair of the Cyshis tag and Ni(II)-Nta probe for highly selective chemical labeling of membrane proteins

Chemical labeling of proteins with synthetic molecular probes offers the possibility to probe the functions of proteins of interest in living cells. However, the methods for covalently labeling targeted proteins using complementary peptide tag-probe pairs are still limited, irrespective of the versatility of such pairs in biological research. Herein, we report the new CysHis tag-Ni(II) probe pair for the specific covalent labeling of proteins. A broad-range evaluation of the reactivity profiles of the probe and the CysHis peptide tag afforded a tag-probe pair with an optimized and high labeling selectivity and reactivity. In particular, the labeling specificity of this pair was notably improved compared to the previously reported one. This pair was successfully utilized for the fluorescence imaging of membrane proteins on the surfaces of living cells, demonstrating its potential utility in biological research.

Protein Delivery System Containing a Nickel-Immobilized Polymer for Multimerization of Affinity-Purified His-Tagged Proteins Enhances Cytosolic Transfer

Recombinant proteins with cytosolic or nuclear activities are emerging as tools for interfering with cellular functions. Because such tools rely on vehicles for crossing the plasma membrane we developed a protein delivery system consisting in the assembly