20545-93-1

Basic information

• Product Name: 5-hydroxy-6-(4-(5'-methylpyrimidine-2'-one)dihydrothymine)
• Synonyms: 5-hydroxy-6-(4-(5'-methylpyrimidine-2'-one)dihydrothymine);4,5-Dihydro-5-hydroxy-5,5'-dimethyl-4,4'-bipyrimidine-2,2',6(1H,1'H,3H)-trione;5-Hydroxy-5-methyl-6-(2-oxo-5-methyl-1,2-dihydropyrimidine-4-yl)hexahydropyrimidine-2,4-dione
• CAS NO: 20545-93-1
• Molecular Formula: C10H12 N4 O4
• Molecular Weight: 252.23
• Mol File: 20545-93-1.mol
• Article Data: 3

Chemical Properties

• Boiling Point: °Cat760mmHg
• Flash Point: °C
• Appearance: /
• Density: 1.7g/cm³
• CAS DataBase Reference: 5-hydroxy-6-(4-(5'-methylpyrimidine-2'-one)dihydrothymine)(CAS DataBase Reference)
• NIST Chemistry Reference: 5-hydroxy-6-(4-(5'-methylpyrimidine-2'-one)dihydrothymine)(20545-93-1)
• EPA Substance Registry System: 5-hydroxy-6-(4-(5'-methylpyrimidine-2'-one)dihydrothymine)(20545-93-1)

Safety Data

• Hazardous Substances Data: 20545-93-1(Hazardous Substances Data)

Upstream product

• 87367-14-4 thymidilyl(3'-5')thymidine 
• 50-89-5 thymidine 
• 41398-83-8 C₂₀H₂₈N₄O₁₀ 

Downstream Products

• 20545-68-0 thyminyl-(6-4')-(5'-methyl-1',2'-dihydropyrimidin-2'-one) 

Relevant articles and documents

Measurement of Pyrimidine (6-4) Photoproducts in DNA by a Mild Acidic Hydrolysis-HPLC Fluorescence Detection Assay

Pyrimidine (6-4) pyrimidone photoproducts constitute one of the major classes of DNA lesions induced by far-UV irradiation. However, their biological role remains difficult to assess partly because of the lack of a specific and sensitive assay for monitoring their formation in DNA. Here is presented a measurement method based on the release of the (6-4) base adducts from DNA followed by an HPLC separation associated with a sensitive and specific fluorescence detection. The quantitative and mechanistic aspects of the chemical hydrolysis, based on the use of hydrogen fluoride stabilized in pyridine, were investigated, using dinucleoside monophosphate (6-4) photoproducts as model compounds. The final hydrolysis products were isolated and characterized by UV, fluorescence, mass, and 1H NMR spectroscopies. Application of the assay to far-UV irrradiated calf thymus DNA provided information on the sequence effect on the rate of formation of three of the four possible bipyrimidine (6-4) photoproducts.