206752-36-5 Usage
Chemical Properties
white fine crystalline powder
Uses
Different sources of media describe the Uses of 206752-36-5 differently. You can refer to the following data:
1. Benzamidine hydrochloride hydrate is a reversible inhibitor of trypsin, trypsin-like enzymes, and serine proteases. It is a proteolytic inhibitor and apoptosis reagent.
2. Benzamidine hydrochloride hydrate has been used:in western blot analysisin immunoprecipitationin the purification of yeast dyneinas a protease inhibitor in the preparation of brush-border membrane vesiclesfor the processing of human synovial fluid, blood and eye sleepto prevent non-specific degradation of cartilages
Application
Benzamidine hydrochloride hydrate has been used:in western blot analysisin immunoprecipitationin the purification of yeast dyneinas a protease inhibitor in the preparation of brush-border membrane vesiclesfor the processing of human synovial fluid, blood and eye sleepto prevent non-specific degradation of cartilages
Biological Activity
Peptidase inhibitor.Benzamidine HCl is a reversible inhibitor of trypsin, trypsin-like enzymes, and serine proteases.A concentration of approximately 1 mM is used for general protease inhibition. To inhibit proteases from yeast, a range of 0.5 to 4.0 mM is used and it is for the most part interchangeable with pepstatin A.In addition to being a strong competitive inhibitor of trypsin, benzamidine HCl has been also shown to be a strong competitive inhibitor of thrombin and plasmin. It was also found to be as effective as aprotinin in the prevention of glucagon degradation in human plasma.
Biochem/physiol Actions
Peptidase inhibitor.
Purification Methods
Recrystallise it from dilute HCl (crystals contain xH2O) or EtOH/few drops HCl; dry it in a vacuum. It is a proteolytic inhibitor [Jeffcoate & White J Clin Endocrinol Metab 38 155 1974, Beilstein 9 IV 898.]
Preparation and handling
Benzamidine HCl is soluble in water and alcohol. Solubility testing in water at a concentration of 50 mg/ml yields a clear solution with heating. Benzamidine HCl is sensitive to oxidation. It is recommended to prepare solutions fresh each time in degassed water prior to use. However, frozen aliquots stored under inert gas, to exclude air, may be stable for a short time. Insufficient information is available to assess the shelf-life of a frozen solution.
Precautions
Keep container tightly sealed. Store in cool, dry conditions in well sealed containers. Incompatible with oxidizing agents. It is hygroscopic in nature.
Check Digit Verification of cas no
The CAS Registry Mumber 206752-36-5 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 2,0,6,7,5 and 2 respectively; the second part has 2 digits, 3 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 206752-36:
(8*2)+(7*0)+(6*6)+(5*7)+(4*5)+(3*2)+(2*3)+(1*6)=125
125 % 10 = 5
So 206752-36-5 is a valid CAS Registry Number.
InChI:InChI=1/C7H8N2/c8-7(9)6-4-2-1-3-5-6/h1-5H,(H3,8,9)/p+1
