207613-94-3Relevant academic research and scientific papers
A conjugate from a laminin-related peptide, Tyr-IIe-Gly-Ser-Arg, and chitosan: Efficient and regioselective conjugation and significant inhibitory activity against experimental cancer metastasis
Nishiyama, Yasuhiro,Yoshikawa, Tomoko,Ohara, Nobumichi,Kurita, Keisuke,Hojo, Kciko,Kamada, Haruhiko,Tsutsumi, Yasuo,Mayumi, Tadanori,Kawasaki, Koichi
, p. 1161 - 1165 (2007/10/03)
A laminin-related antimetastatic peptide was conjugated with chitosan, and antimetastatic activity of the peptide-chitosan conjugate was assayed. Chitosan was converted to its organosoluble derivative, 6-O-trityl-chitosan, in 3 steps, and then coupled wit
Preparation of a chitosan hybrid of an antimetastatic laminin-related peptide
Hojo, Keiko,Maeda, Mitsuko,Mu, Yu,Kamada, Haruhiko,Tsutsumi, Yasuo,Nishiyama, Yasuhiro,Yoshikawa, Tomoko,Kurita, Keisuke,Block, Lawrence H.,Mayumi, Tadanori,Kawasaki, Koichi
, p. 277 - 280 (2007/10/03)
A hybrid of chitosan and an antimetastatic laminin-related peptide was prepared. Acetyl-Tyr-Ile-Gly-Ser-Arg-βAla-OH (Ac-YIGSRβA-OH) was prepared by a solid-phase method and reacted with a water-soluble chitosan. Chitosan amino groups did not react with the peptide using diphenylphosphoryl azide, diisopropylcarbodiimide/1-hydroxybenzotriazole, water-soluble carbodiimide/1-hydroxybenzotriazole, phosphazo, or 2-(1 H-benzotriazole-1-y1)-1,1,3,3-tetramethyluronium tetrafluoroborate (TBTU) methods. A small spacer was therefore put between the peptide and the chitosan. tert-Butyloxycarbonyl-Gly (spacer) was reacted with chitosan by the TBTU method. After removal of the protecting group, the Gly-chitosan was coupled with Ac-YIGSRβA-OH by the water-soluble carbidiimide method to give Ac-YIGSRβAG-chitosan. The inhibitory effect of the peptide-chitosan hybrid on experimental metastasis in mice was not reduced, but actually potentiated, suggesting that chitosan may be used as a drug carrier for peptides.
