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Usage

Uses

Used in Biomedical Research:
FLASH is used as a fluorescence labeling agent for the detection and analysis of protein expression and interactions in living cells. This application is particularly useful in biomedical research for studying protein dynamics, cellular processes, and the effects of various treatments on protein behavior.
Used in Drug Delivery Systems:
In the field of drug delivery, FLASH can be used as a component in the development of novel drug delivery systems. The unique properties of the dye can be exploited to improve the targeting, release, and monitoring of therapeutic agents within the body.
Used in Analytical Chemistry:
FLASH can also be employed in analytical chemistry as a tool for detecting and quantifying specific proteins or other biomolecules. Its fluorescence properties make it an ideal candidate for high-throughput screening and other analytical applications.
Used in Diagnostics:
The fluorescence labeling capabilities of FLASH can be applied in the development of diagnostic tools for various diseases and conditions. By targeting specific proteins or biomarkers associated with a particular disease, FLASH can help in the early detection and monitoring of disease progression.
Used in Pharmaceutical Development:
In the pharmaceutical industry, FLASH can be utilized for the identification and optimization of drug candidates. Its ability to label and track proteins can aid in understanding the mechanisms of action, efficacy, and potential side effects of new drugs.
Used in Environmental Monitoring:
FLASH can be employed in environmental monitoring to detect and quantify specific proteins or biomarkers associated with pollution or other environmental stressors. This can help in assessing the impact of human activities on ecosystems and developing strategies for environmental protection and remediation.

InChI:InChI=1/C24H18As2O5S4/c27-17-7-5-15-19(13-3-1-2-4-14(13)24(29)30)16-6-8-18(28)21(26-34-11-12-35-26)23(16)31-22(15)20(17)25-32-9-10-33-25/h1-8,27H,9-12H2,(H,29,30)

Upstream product

• 943517-73-5 fluorescein-4',5'-bis(mercuric trifluoroacetate) 
• 540-63-6 ethane-1,2-dithiol 

Relevant academic research and scientific papers

Improved photostable FRET-competent biarsenical-tetracysteine probes based on fluorinated fluoresceins

We have developed fluoro-substituted versions of the biarsenical-tetracysteine label FlAsH, exhibiting significant improvements in important properties over the original fluorescein derivative. In complexes with tetracysteine targets, F2FlAsH exhibits 50 times improved photostability, lower pH sensitivity, higher absorbance and quantum yield than FlAsH, and F4FlAsH adds a new color to the palette of biarsenical dyes. The two probes also provide a new FRET pair with a larger Ro value (54 A) than any previously obtained with biarsenical dyes. Copyright

Tetracysteine-tagged prion protein allows discrimination between the native and converted forms

The conformational conversion of prion protein (PrP) from a native conformation to the amyloid form is a hallmark of transmissible spongiform encephalopathies. Conversion is usually monitored by fluorescent dyes, which bind generic amyloids and are less suited for living cell imaging. We report a new method for the synthesis of membrane-permeable and membrane-impermeable biarsenical reagents, which are then used to monitor murine PrP (mPrP) misfolding. We introduced tetracysteine (TC) tags into three different positions of mPrP, which folded into a native-like structure. Whereas mPrPs with a TC tag inserted at the N-terminus or C-terminus supported fibril formation, insertion into the helix 2-helix 3 loop inhibited conversion. We devised a quantitative protease-free method to determine the fraction of converted PrP, based on the ability of the fluorescein arsenical helix binder reagent to differentiate between the monomeric and fibrilized form of TC-tagged PrP, and showed that TC-tagged mPrP could be detected on transfected cells, thereby expanding the potential use of this method for the detection and study of conformational diseases.

IMPROVED PHOTOSTABLE FRET-COMPETENT BIARSENICAL-TETRACYSTEINE PROBES BASED ON FLUORINATED FLUORESCEINS

A fluorogenic dye having the formula (I) or (II) and salts of said fluorogenic dyes.