212832-35-4

Upstream product

• 40615-36-9 4,4'-dimethoxytrityl chloride 
• 951-77-9 2'-Deoxycytidine 
• 51432-39-4 N⁴,O^3'_,O5'-tris-trimethylsilanyl-2'-deoxy-cytidine 
• 102434-71-9 2'-deoxy-3',5'-di-O-1,1,3,3-(tetraisopropyldisiloxane-1,3-diyl)cytidine 

Downstream Products

• 630109-91-0 Carbonic acid (2R,3S,5R)-5-(4-{[bis-(4-methoxy-phenyl)-phenyl-methyl]-amino}-2-oxo-2H-pyrimidin-1-yl)-3-hydroxy-tetrahydro-furan-2-ylmethyl ester 3-trifluoromethyl-phenyl ester 
• 146691-39-6 N⁴-dimethoxytrityl-5'-amino-2',5'-dideoxcytidine 
• 80594-29-2 1-{(2R,4S,5R)-5-[Bis-(4-methoxy-phenyl)-phenyl-methoxymethyl]-4-hydroxy-tetrahydro-furan-2-yl}-4-{[bis-(4-methoxy-phenyl)-phenyl-methyl]-amino}-1H-pyrimidin-2-one 

Relevant academic research and scientific papers

Solid-Phase Oligodeoxynucleotide Synthesis: A Two-Step Cycle Using Peroxy Anion Deprotection

A novel solid-phase phosphoramidite based oligodeoxynucleotide two-step synthesis method has been developed. Keys to this method are replacement of the 5′-dimethoxytrityl blocking group with an aryloxycarbonyl and the use of N-dimethoxytrityl protection for the exocyclic amines of adenine and cytosine. With these modifications, coupling of each 2′-deoxynucleoside 3′-phosphoramidite to the growing oligodeoxynucleotide on the solid support can be followed by treatment with an aqueous mixture of peroxy anions buffered at pH 9.6. This reagent effectively removes the carbonate protecting group and simultaneously oxidizes the phosphite internucleotide linkage. As a consequence a new two-step synthesis cycle is possible. Oligodeoxynucleotides synthesized using this approach are identical to authentic samples when tested by a variety of analytical techniques.

Functionalization of solid supports with N-unprotected deoxyribonucleosides

N-Unprotected deoxyribonucleotides were loaded to solid supports via a succinyl or an oxalyl linker. These solid supports were successfully used for oligonucleotide synthesis by the H-phosphonate method without N-protecting groups.

Synthesis, Enzymatic Stability And Physicochemical Properties Of Oligonucleotides Containing A N-Cyanoguanidine Linkage.

Nucleoside dimers with a N-cyanoguanidine linkage were synthesized and used as building blocks for oligonucleotide synthesis.Oligonucleotides composed of alternating phosphodiester and cyanoguanidine functions are still able to hybridize with a complementary natural oligodeoxynucleotide.