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2',4',5',7'-Tetrabromo-3,4,5,6-tetrachlorofluorescein, commonly known as eosin Y, is a synthetic red fluorescent dye that belongs to the class of xanthene dyes. It is characterized by a tetrachlorofluorescein core with four bromine substituents, which imparts its distinctive red color and enables its use as a fluorochrome in various scientific and medical applications.

2134-15-8

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2134-15-8 Usage

Uses

Used in Histological Staining:
2',4',5',7'-TETRABROMO-3,4,5,6-TETRACHLOROFLUORESCEIN is used as a histological stain for both medical and biological research. It aids in the visualization of tissues and cells under a microscope, providing contrast and enhancing the identification of cellular structures.
Used in Cell Viability Assays:
In laboratory applications, 2',4',5',7'-TETRABROMO-3,4,5,6-TETRACHLOROFLUORESCEIN serves as a vital component in cell viability assays. It helps in determining the proportion of living cells in a sample, which is crucial for assessing the effects of treatments or conditions on cell populations.
Used in Fluorescence Microscopy:
2',4',5',7'-TETRABROMO-3,4,5,6-TETRACHLOROFLUORESCEIN is utilized as a fluorochrome in fluorescence microscopy. Its red fluorescence allows for the detection and imaging of specific cellular components or processes, contributing to a deeper understanding of cell biology.
Used as a Counterstain in Combination with Hematoxylin:
In the field of histology, 2',4',5',7'-TETRABROMO-3,4,5,6-TETRACHLOROFLUORESCEIN is used as a counterstain alongside hematoxylin. This combination enhances the contrast between different types of tissues and cells, facilitating the identification and analysis of various cellular structures during microscopic examination.

Check Digit Verification of cas no

The CAS Registry Mumber 2134-15-8 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 2,1,3 and 4 respectively; the second part has 2 digits, 1 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 2134-15:
(6*2)+(5*1)+(4*3)+(3*4)+(2*1)+(1*5)=48
48 % 10 = 8
So 2134-15-8 is a valid CAS Registry Number.
InChI:InChI=1/C20H4Br4Cl4O5/c21-5-1-3-17(9(23)15(5)29)32-18-4(2-6(22)16(30)10(18)24)20(3)8-7(19(31)33-20)11(25)13(27)14(28)12(8)26/h1-2,29-30H

2134-15-8SDS

SAFETY DATA SHEETS

According to Globally Harmonized System of Classification and Labelling of Chemicals (GHS) - Sixth revised edition

Version: 1.0

Creation Date: Aug 15, 2017

Revision Date: Aug 15, 2017

1.Identification

1.1 GHS Product identifier

Product name 2',4',5',7'-Tetrabromo-4,5,6,7-tetrachloro-3',6'-dihydroxy-3H-spi ro[2-benzofuran-1,9'-xanthen]-3-one

1.2 Other means of identification

Product number -
Other names 2-hydroxynonachlorodiphenyl ether

1.3 Recommended use of the chemical and restrictions on use

Identified uses For industry use only.
Uses advised against no data available

1.4 Supplier's details

1.5 Emergency phone number

Emergency phone number -
Service hours Monday to Friday, 9am-5pm (Standard time zone: UTC/GMT +8 hours).

More Details:2134-15-8 SDS

2134-15-8Synthetic route

1',2',3',4'-tetrachlorofluorescein
13245-63-1

1',2',3',4'-tetrachlorofluorescein

2,4,5,7-tetrabromo-1',2',3',4'-tetrachlorofluorescein
2134-15-8

2,4,5,7-tetrabromo-1',2',3',4'-tetrachlorofluorescein

Conditions
ConditionsYield
With bromine; sodium carbonate In ethanol for 12h; Ambient temperature;78%
tetrachlorophthalic anhydride
117-08-8

tetrachlorophthalic anhydride

2,4,5,7-tetrabromo-1',2',3',4'-tetrachlorofluorescein
2134-15-8

2,4,5,7-tetrabromo-1',2',3',4'-tetrachlorofluorescein

Conditions
ConditionsYield
Multi-step reaction with 2 steps
1: 60 percent / 2.5 h / 200 °C
2: 78 percent / Br2, 5percent aq. Na2CO3 / ethanol / 12 h / Ambient temperature
View Scheme
recorcinol
108-46-3

recorcinol

2,4,5,7-tetrabromo-1',2',3',4'-tetrachlorofluorescein
2134-15-8

2,4,5,7-tetrabromo-1',2',3',4'-tetrachlorofluorescein

Conditions
ConditionsYield
Multi-step reaction with 2 steps
1: 60 percent / 2.5 h / 200 °C
2: 78 percent / Br2, 5percent aq. Na2CO3 / ethanol / 12 h / Ambient temperature
View Scheme
1,10-Phenanthroline
66-71-7

1,10-Phenanthroline

2,4,5,7-tetrabromo-1',2',3',4'-tetrachlorofluorescein
2134-15-8

2,4,5,7-tetrabromo-1',2',3',4'-tetrachlorofluorescein

cadmium(II)

cadmium(II)

{Cd(C12H8N2)2}(2+)*Cl4Br4C20H2O5(2-)={Cd(C12H8N2)2}Cl4Br4C20H2O5

{Cd(C12H8N2)2}(2+)*Cl4Br4C20H2O5(2-)={Cd(C12H8N2)2}Cl4Br4C20H2O5

Conditions
ConditionsYield
In water addn. of acetate-ammonia buffer soln. (pH = 3 - 10), 1E-2 M phenanthroline soln. and 1E-2 M fluorescein soln. to aq. soln. of Cd; mixt. was transferred to separatory funnel; addn. of CHCl3; shaken 1 min; sepn. of phases; addn. of acetone to CHCl3 layer; not isolated; only in soln.; detn. by fluorescence spectroscopy;;
1,10-Phenanthroline
66-71-7

1,10-Phenanthroline

2,4,5,7-tetrabromo-1',2',3',4'-tetrachlorofluorescein
2134-15-8

2,4,5,7-tetrabromo-1',2',3',4'-tetrachlorofluorescein

zinc(II) cation

zinc(II) cation

{Zn(C12H8N2)2}(2+)*Cl4Br4C20H2O5(2-)={Zn(C12H8N2)2}Cl4Br4C20H2O5

{Zn(C12H8N2)2}(2+)*Cl4Br4C20H2O5(2-)={Zn(C12H8N2)2}Cl4Br4C20H2O5

Conditions
ConditionsYield
In water addn. of acetate-ammonia buffer soln. (pH = 3 - 10), 1E-2 M phenanthroline soln. and 1E-2 M fluorescein soln. to aq. soln. of Zn; mixt. was transferred to separatory funnel; addn. of CHCl3; shaken 1 min; sepn. of phases; addn. of acetone to CHCl3 layer; not isolated; only in soln.; detn. by fluorescence spectroscopy;;

2134-15-8Downstream Products

2134-15-8Relevant academic research and scientific papers

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