220036-88-4Relevant academic research and scientific papers
7-the to nitrogen -2 [...] -deoxy-7-halogen-substituted guanine nucleoside synthesis method
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Paragraph 0049; 0050; 0051, (2017/02/24)
The invention discloses a synthetic method of 7-denitrified-2'-deoxidized-7-halogen substituted guanosine; the method comprises the following steps: a compound in the following formula (I) is obtained from a compound in the following formula (V) by demeth
Synthesis of acid-sensitive connection unit and its use in DNA sequencing
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Paragraph 0476; 0485; 0491; 0492, (2018/02/04)
The invention discloses a synthesis method of an acid sensitive connection unit, and a use of the acid sensitive connection unit in DNA sequencing. The structural formula of the acid sensitive connection unit is shown in the specification. In the structural formula, R is NH2 or N3, m is an integer from 0 to 44, and n is an integer from 0 to 44; R1 and R2 respectively represent an aliphatic alkyl group, or R1 and R2 respectively represent an aromatic derivative, or R1 is a phenyl group, a naphthyl group, a phenyl derivative or a naphthyl derivative, and R2 is an aliphatic alkyl group or hydrogen; or R2 is a phenyl group, naphthyl group, a phenyl derivative or a naphthyl derivative, R1 is an aliphatic alkyl group or hydrogen, or R1 and R2 form a cyclohexyl group, a cyclopentyl group or a cyclobutyl group. A reversible terminal obtained through connecting the acid sensitive connection unit with nucleotide and fluorescein can be used in DNA sequencing-by-synthesis. The reversible terminal can be used in the DNA sequencing; and raw materials required by the synthesis method are simple and can be easily obtained, and the synthesis process is a routine chemical reaction, so the method can realize large scale popularization use.
SYNTHESIS OF FOUR COLOR 3'-O-ALLYL MODIFIED PHOTOCLEAVABLE FLUORESCENT NUCLEOTIDES AND RELATED METHODS
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Page/Page column Sheet 7/22; 40, (2010/11/27)
This invention provides a process for making 3'-O- allyl-dGTP-PC-Biodopy-FL-51O, 3' -0-allyl-dATP-PC-ROX, 3'-O-allyl-dCTP-PC-Bodipy-650 and 3' -O-allyl-dUTP-PC- R6G, and related methods.
Design and synthesis of a photocleavable fluorescent nucleotide 3′-O-allyl-dGTP-PC-Bodipy-FL-510 as a reversible terminator for DNA sequencing by synthesis
Meng, Qinglin,Kim, Dae Hyun,Bai, Xiaopeng,Bi, Lanrong,Turro, Nicholas J.,Ju, Jingyue
, p. 3248 - 3252 (2007/10/03)
DNA sequencing by synthesis (SBS) using reversible fluorescent nucleotide terminators is potentially an efficient approach to address the limitations of current DNA sequencing techniques. Here, we report the design and synthesis of a 3′-O-allyl photocleav
