22356-88-3

Upstream product

• 108-77-0 1,3,5-trichloro-2,4,6-triazine 
• 81-69-6 1-amino-4-(4-amino-3-sulfophenylamino)-9,10-dioxo-9,10-dihydroanthracene-2-sulfonic acid 
• 116-81-4 1-amino-4-bromo-9,10-dioxoanthracene-2-sulphonic acid 
• 88-45-9 2,5-diaminobenzenesulfonic acid 

Downstream Products

• 72696-54-9 reactive blue 2 

Relevant academic research and scientific papers

METHOD FOR PURIFICATION OF ANTIBODIES, ANTIBODY FRAGMENTS OR ENGINEERED VARIANTS THEREOF USING SPECIFIC ANTHRAQUINONE DYE-LIGAND STRUCTURES

The present invention relates to novel adsorbents applicable a process for the separation or purification of antibodies, antibody fragments or engineered variants thereof, which comprise anthraquinone dye ligands; corresponding purification processes; and corresponding analytical or preparative separation kits.

ADSORBENTS COMPRISING ANTHRAQUINONE DYE-LIGANDS FOR THE SEPARATION OF BIOLOGICAL MATERIALS

A process for the separation of biological materials, wherein an adsorbent is used, which comprises a reaction product of a compound of formula and a substrate having a group capable of reaction with a reactive group in said compound of formula (1) to for

Constitutional isomers of Reactive Blue 2 - Selective P2Y-receptor antagonists?

The anthraquinone derivative Reactive Blue 2 (RB 2) is one of the most widely used P2-receptor antagonists, still claimed to be P2Y-selective. RB 2 is defined as a mixture of two constitutional isomers and commercially available in different identity and purity. A sample of RB 2, offered for sale by RBI, purchased from Biotrend, Koeln, Germany, was chromatographically purified and identified by 1H- and 13C-NMR studies as a 35:65 mixture of the terminal ring F meta and para constitutional isomers. The two constitutional isomers of RB 2 were synthesised and tested alongside with the ortho isomer Cibacron Blue 3GA (CB 3GA) on contractions of the rat vas deferens (RVD) elicited by α,β-methylene ATP (α,β-MeATP), mediated by P2X1-receptors, and relaxations of the carbachol-precontracted guinea pig taenia coli elicited by adenosine 5′-O-(2-thiophosphate) (ADPβS), mediated by P2Y1-like-receptors. All compounds inhibited the α,β-MeATP induced contraction of the RVD and the ADPβS induced relaxation of the carbachol precontracted guinea-pig taenia coli. The IC50-values at P2X1-R were 9.1 μM for CB 3GA, 28.4 μM for RB 2, 19.7 μM for RB 2 meta, and 35.5 μM for RB 2 para. The IC50-values at P2Y1-like-R were 17.4 μM for CB 3GA, 7.7 μM for RB 2, 12.0 μM for RB 2 meta, and 2.6 μM for RB 2 para. The results clearly show that neither RB 2 as a mixture nor the pure ortho and meta isomer are P2Y1-like- versus P2X1-selective antagonists. In contrast the pure para-isomer of RB 2 is a moderately P2Y1-like- versus P2X1-selective antagonist.