22596-06-1Relevant academic research and scientific papers
Utilization method of waste alpha-1,2,3,5-tetra-O-acetyl-D-ribose in ribose crystalline residual liquid
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Paragraph 0054-0056; 0060; 0064; 0068; 0072; 0076; 0086, (2017/05/27)
The invention relates to a method for utilizing waste in ribose crystalline residual liquid. The method comprises the steps that crystalline residual liquid obtained after nucleoside pyrolysis serves as a raw material, an organic layer is extracted, an organic solvent is vaporized and eliminated, mixing is conducted with benzotriazole, under the action of a catalyst, on the condition that the temperature ranges from 100 DEG C to 160 DEG C, a condensation reaction is conducted for 0.33-5.0 h, after the reaction is completed, ethyl acetate is added for dissolving, washing is conducted with water, the solvent is dried out, recrystallization is conducted with diethyl ether, an intermediate product 1-(2,3,5-tri-O-acetyl-beta-D-furanribosyl)benzotriazole is obtained, under the faction of an aminomethanol solution or a sodium methylate methanol solution, acetyl is removed at room temperature, and 1-(2,3,5-tri-hydroxyl-beta-D-furanribosyl)benzotriazole is obtained. The method has the prominent advantages the waste is turned into wealth, at the same time of reducing beta-tetra-O-acetyl-D-ribose crystalline residual liquid, the original waste alpha-tetra-O-acetyl-D-ribose is utilized, and the method has the obvious implement value and social and economic benefits.
Synthesis and enzymatic activity of some new purine ring system analogues of adenosine 3',5'-cyclic monophosphate
Sagi,Szucs,Vereb,Otvos
, p. 4549 - 4556 (2007/10/02)
A series of novel adenosine 3',5'-cyclic monophosphate (cAMP) analogues, as well as their 6-deamino and 6-nitro derivatives, were synthesized where the purine ring was replaced by indazole, benzotriazole, and benzimidazole. The 3',5'-cyclic monophosphates of indazole and benzotriazole ribofuranosides, where the sugar-phosphate moiety is attached to the N-2 nitrogen atoms of the heterocycles, were also prepared. The biological efficiency of the analogues was tested by their ability to activate purified cAMP-dependent protein kinase I (PK-I) from rabbit skeletal muscle and cAMP- dependent protein kinase II (PK-II) from bovine heart. Each cyclic nucleotide is capable of activating both PK isozymes in half-maximum concentrations (K(a)) ranging from 2.0 · 10-8 to 4.8 · 10-6 M. The cyclic phosphate of N-1-β-D-ribofuranosylindazole (13) proved to be a very poor activator for both PK-I and PK-II, but when indazole binds by N-2 to ribose or when the hydrogen atom at C-4 is substituted by a nitro or amino group, activities of the analogues increase considerably. The activating potencies of benzotriazole derivatives are similar to that of cAMP, irrespective of the C- 4 substituents. The K(a)' values of cyclic nucleotides containing benzimidazole were found to be higher for PK-II than for PK-I; e.g. the activity of 4-nitro-1-β-D-ribofuranosylbenzimidazole 3',5'-cyclic monophosphate (32) is nearly 20 times as high for PK-II than for PK-I.
Enzymatic ADP-ribosylation of Benzotriazoles and Related Triazoles. Difference of Glycosidation Site between Triazoles and Indazoles
Tono-oka, Shuichi,Azuma, Ichiro
, p. 339 - 343 (2007/10/02)
Some benzotriazoles and related triazole were ADP-ribosylated in the presence of NAD and NAD-nucleosidase to give corresponding dinucleotides.The site of ribosylation for these dinucleotides was found exclusively to be N-1 on the basis of their uv and 15N-nmr spectra, contrasting with N-2 for indazole dinucleotides.The origin of this difference in regiochemical specificity between the triazole and diazole systems is discussed.
