23555-00-2

Basic information

• Product Name: HOECHST 34580
• Synonyms: HOECHST 34580;HOE 34580;ProaMine;2,50-bibenzimidazole, 20-[p-(dimethylamino)phenyl]-5-(4-methyl-1-piperazinyl)-
• CAS NO: 23555-00-2
• Molecular Formula: C₂₇H₂₉N₇
• Molecular Weight: 451.56606
• Product Categories: Benzimidazole
• Mol File: 23555-00-2.mol
• Article Data: 3

Chemical Properties

• Melting Point: >300℃
• Appearance: Yellow to green/powder
• Storage Temp.: 2-8°C(protect from light)
• Solubility: Soluble in dimethyl sulfoxide, water
• PKA: 10.97±0.10, most acidic, temperature: 25 °C; 7.66 ± 0.42, most basic(at 25℃)
• CAS DataBase Reference: HOECHST 34580(CAS DataBase Reference)
• NIST Chemistry Reference: HOECHST 34580(23555-00-2)
• EPA Substance Registry System: HOECHST 34580(23555-00-2)

Safety Data

• Hazardous Substances Data: 23555-00-2(Hazardous Substances Data)

Usage

Description

HOECHST 34580 is a fluorescent dye that is widely utilized in cell biology and neuroscience research. It is characterized by its blue emission and its ability to bind specifically to DNA, making it an indispensable tool for staining cell nuclei in both live and fixed cells. The dye's high specificity for DNA and its low cytotoxicity have contributed to its popularity in fluorescent imaging studies, particularly in the realms of cell division and cell cycle progression. Furthermore, HOECHST 34580 has been instrumental in the development of diagnostic tools for the identification and quantification of DNA in a variety of biological samples.

Uses

Used in Cell Biology Research:
HOECHST 34580 is used as a fluorescent stain for visualizing cell nuclei, aiding researchers in the study of cellular and subcellular structures. Its application is crucial for observing and analyzing cell division and cell cycle progression due to its ability to bind to DNA and emit a blue fluorescence.
Used in Neuroscience Research:
In the field of neuroscience, HOECHST 34580 serves as a vital tool for staining and visualizing the nuclei of neurons, contributing to the understanding of neural development, connectivity, and function.
Used in Diagnostic Tool Development:
HOECHST 34580 is used as a component in the development of diagnostic tools for identifying and quantifying DNA in various biological samples. Its high specificity and fluorescence properties make it suitable for accurate and sensitive DNA detection.
Used in Organelle Labeling:
HOECHST 34580 is also used to label DNA in organelles such as mitochondria, providing insights into mitochondrial DNA replication, transcription, and its role in cellular processes.

Upstream product

• 6393-40-4 4-Amino 3-nitro-benzonitrile 
• 158667-64-2 2-(4-dimethylaminophenyl)-1H-benzimidazole-5-carbonitrile 
• 17626-40-3 4-cyano-1,2-phenylenediamine 
• 54998-08-2 4-(4-methylpiperazino)-1,2-diaminobenzene 
• 100-10-7 4-dimethylamino-benzaldehyde 
• 23491-49-8 2-amino-4-<5'-(4''-methylpiperazin-1''-yl)benzimidazol-2'-yl>aniline 
• 696581-55-2 4-dimethylamino-1-[5'-imino(ethoxy)methylbenzimidazo-2'-yl]benzene hydrochloride 

Relevant articles and documents

Radioprotectors

A compound of the formula (Ib): wherein X is NCH3, Y is N, Z is N, R3 is N(CH3)2, and (a) R1 is CH3, R2, R4 and R5 to R11 are hydrogen or (b) R5 is CH3 and R1, R2, R4 and R6 to R11 are hydrogen, and salts and tautomers thereof.

Basic-substituted 2,6-bisbenzimidazole derivates, a novel class of substances with chemotherapeutic activity

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