23664-77-9Relevant academic research and scientific papers
The N-Phosphobiotin Route: a Possible New Pathway for Biotin Coenzyme
Blonski, Casimir,Belghith, Hachemi,Klaebe, Alain,Perie, Jean-Jacques
, p. 1369 - 1374 (2007/10/02)
The formation of N-acylureas from N-phosphorylated ureas under very mild conditions is presented as a possible route to the formation of carboxybiotin from biotin in biotin-mediated enzymatic carboxylations in which an N-phosphobiotin is the activated form.The detailed mechanistic pathway for the model reaction indicates that an intermediate acylphosphate is formed, the rate-determining step being a nucleophilic attack of the ureido anion or its enol on this acylphosphate.In no case was an O-phosphorylated intermediate (of the phosphobiotin type) involved.
