254109-22-3 Usage
Uses
Used in Research and Diagnostic Applications:
4-Amino-5-methylamino-2',7'-difluorofluoresceindiacetate is used as a quantification reagent for detecting and measuring low concentrations of nitric oxide (NO) in solutions. Its application is crucial in understanding the role of NO in various biological processes and its involvement in different diseases.
Used in Tissue Analysis:
In the field of biomedical research, 4-Amino-5-methylamino-2',7'-difluorofluoresceindiacetate is used as an analytical tool for measuring nitric oxide (NO) levels in liver tissue homogenates. This helps researchers and medical professionals to study the role of NO in liver function and its potential implications in liver-related diseases.
Used in Cellular and Molecular Biology:
4-Amino-5-methylamino-2',7'-difluorofluoresceindiacetate is employed as a staining agent for the detection of nitric oxide (NO) from human umbilical vein endothelial cells (HUVECs). This application aids in understanding the cellular mechanisms involving NO and its impact on endothelial cell function, which is vital for various physiological processes and disease progression.
Biochem/physiol Actions
DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM and DAF-FM DA are more sensitive to nitric oxide (NO) compared with DAF-2 and more photo-stable and less pH sensitive. DAF-FM DA permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases.
Check Digit Verification of cas no
The CAS Registry Mumber 254109-22-3 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 2,5,4,1,0 and 9 respectively; the second part has 2 digits, 2 and 2 respectively.
Calculate Digit Verification of CAS Registry Number 254109-22:
(8*2)+(7*5)+(6*4)+(5*1)+(4*0)+(3*9)+(2*2)+(1*2)=113
113 % 10 = 3
So 254109-22-3 is a valid CAS Registry Number.
