25521-23-7Relevant academic research and scientific papers
Isolation, purification and structural characterization of a water-soluble polysaccharide HM41 from Halenia elliptica D. Don
Liu, Chun-Lan,Li, Yang,Xu, Gui-Yun,Li, Ya-Shuang
, p. 979 - 983 (2016/06/13)
A water-soluble polysaccharide, HM41, was obtained from Halenia elliptica D. Don by acidic ethanol fractionation and gel filtration. Its homogeneity was confirmed by chromatography using multiple systems. HM41 was composed of rhamnose (Rha), arabinose (Ara), xylose (Xyl), mannose (Man), galactose (Gal), glucose (Glc) with a molar ratio of 1.0:5.5:1.8:3.0:9.4:21. The average molecular weight of HM41 was approximately 1.17 × 104. Periodate oxidation, Smith degradation, methylation and GC, IR, NMR, XRD, GC-MS analysis were used for the structural analysis of HM41. Its main chain was composed mainly of β-(1 → 4)Gal, β-(1 → 4)Glc and β-(1 → 6)Glc. β-(1 → 4)Gal were substituted at 6-O and on average there were 14 branches among 23 main chain residues; (1 → 4)Glc had no branch; (1 → 6)Glc were substituted at 3-O and on average there were 9 branches among 14 main chain residues. The side chain was composed of (1 → 3,6)-Rha, (1 → 4)/(1 → 5)-Ara, (1 → 4)/(1 → 5)-Xyl, (1 → 4,6)-Man and (1 → 2)-Glc. The terminal residue was composed of Ara, Xyl, Man, Gal, and Glc. Then, we demonstrated that HM and HM41 had strong scavenging activities in vitro hydroxyl. Overall, HM and HM41 may have potential applications in the antioxidants for medical and food industry.
Water-soluble polysaccharides from finger citron fruits (Citrus medica L. var. sarcodactylis)
He, Zhengchun,Liang, Fengjie,Zhang, Yuyang,Pan, Yuanjiang
, p. 100 - 104 (2014/04/03)
Four water-soluble polysaccharides, FCp-1, FCp-2, FCp-3, and FCp-4 were obtained from finger citron fruits (Citrus medica L. var. sarcodactylis) by hot-water extraction and ethanol precipitation, followed by routine separation procedure. Based on the calibration curve, molecular weights of them were estimated to be 113.9, 32.6, 140.3, and 177.1 kDa respectively. The acid hydrolysis, methylation, IR, GC-MS, and NMR experiments were used for composition analysis. FCp-1 was a heteropolysaccharide composed of arabinose, galactose, glucose, rhamnose, and xylose, with a molar ratio of 3.0:7.0:4.1:1.0:1.5. FCp-2 and FCp-4 were →4)-α-d-GalpA(1→ linking galacturonan differ in molecular weights. FCp-3 was a →6)-α-d-Glcp(1→ linking glucan. According to the results of in vitro assays, FCp-3 showed significantly and moderately enhancing capacities toward the proliferation of splenocytes and thymocytes respectively. Thus, FCp-3 or analogs may have further use as immunomodulatory agents.
A facile method for the synthesis of partially O-methylated alditol acetate standards for GC-MS analysis of galactofuranose-containing structures
He, Jian-Yu,Guo, Yu-Na,Zhang, Ling-Ling,Huang, Lin-Hong
, p. 18 - 20 (2013/10/21)
Mixtures of partially O-methylated alditol acetate standards of galactofuranose were synthesized rapidly. Methyl galactofuranosides were obtained with a yield of 79.9% within 4 h under optimized reaction conditions. Methylation of methyl glycosides was carried out in the presence of BaO/Ba(OH)2·8H2O, giving rise to mixtures of partially methylated glycosides. The batch containing the most diverse structures of methyl ethers was converted into partially O-methylated alditol acetates (PMAAs) and then subjected to GC-MS. These PMAAs could be used as GC-MS standards for simultaneous identification of galactofuranose units with diverse linkages in complex carbohydrates.
