25775-99-9 Usage
Uses
Used in Biochemical Research:
2,4-Dinitrophenyl β-D-Galactoside Tetraacetate is used as a substrate for studying the mechanism of spontaneous β-glycoside hydrolysis. The compound is particularly useful in investigating the activity of glycosidases, which are enzymes that catalyze the hydrolysis of glycosidic bonds in carbohydrates. By using 2,4-Dinitrophenyl β-D-Galactoside Tetraacetate, researchers can gain insights into the catalytic mechanisms of these enzymes and their role in various biological processes.
Used in Enzyme Assays:
In the field of enzyme kinetics, 2,4-Dinitrophenyl β-D-Galactoside Tetraacetate is employed as a colorimetric substrate for the quantitative measurement of glycosidase activity. The release of the 2,4-dinitrophenol group upon enzymatic hydrolysis results in a colorimetric change, allowing for the determination of enzyme activity and the study of enzyme kinetics.
Used in Drug Development:
2,4-Dinitrophenyl β-D-Galactoside Tetraacetate can also be used in the development of new drugs targeting glycosidase enzymes. By understanding the interactions between 2,4-Dinitrophenyl β-D-Galactoside Tetraacetate and the enzymes, researchers can design inhibitors or activators that modulate enzyme activity, potentially leading to the development of therapeutic agents for various diseases.
Used in Analytical Chemistry:
In analytical chemistry, 2,4-Dinitrophenyl β-D-Galactoside Tetraacetate can be employed as a reference compound for the calibration of instruments used in the analysis of carbohydrates and related compounds. Its well-defined structure and properties make it a suitable standard for validating analytical methods and ensuring accurate measurements.
Used in Material Science:
Although not directly mentioned in the provided materials, 2,4-Dinitrophenyl β-D-Galactoside Tetraacetate could potentially be used in the development of novel materials with specific properties, such as those with enhanced stability or selectivity in chemical reactions. Its unique structure and functional groups may allow for the creation of new materials with applications in various industries, including pharmaceuticals, agriculture, and environmental science.
Check Digit Verification of cas no
The CAS Registry Mumber 25775-99-9 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 2,5,7,7 and 5 respectively; the second part has 2 digits, 9 and 9 respectively.
Calculate Digit Verification of CAS Registry Number 25775-99:
(7*2)+(6*5)+(5*7)+(4*7)+(3*5)+(2*9)+(1*9)=149
149 % 10 = 9
So 25775-99-9 is a valid CAS Registry Number.
25775-99-9Relevant academic research and scientific papers
The role of sugar substituents in glycoside hydrolysis
Namchuk, Mark N.,McCarter, John D.,Becalski, Adam,Andrews, Trevor,Withers, Stephen G.
, p. 1270 - 1277 (2007/10/03)
A series of monosubstituted deoxy and deoxyfluoro 2,4-dinitrophenyl (DNP) β-D-glycopyranosides was synthesized and used to probe the mechanism of spontaneous β-glycoside hydrolysis. Their relative rates of hydrolysis followed the order 2-deoxy > 4-deoxy > 3-deoxy ? 6-deoxy > parent > 6-deoxy- 6-fluoro > 3-deoxy-3-fluoro > 4-deoxy-4-fluoro > 2-deoxy-2-fluoro. Hammett correlations of the pH-independent hydrolysis rates of each of the 6-, 4-, 3- , and 2-position substituted glycosides with the σ1 value for the sugar ring substituent were linear (r = 0.95 to 0.999, π(I) = -2.2 to -10.7), consistent with hydrolysis rates being largely dictated by field effects on an electron-deficient transition state. The relative rates of hydrolysis of the DNP glucosides can be rationalized on the basis of the stabilities of the oxocarbenium ion-like transition states, as predicted by the Kirkwood- Westheimer model. The primary determinant of the rate of hydrolysis within a series appears to be the field effect of the ring substituent on O5, the principal center of charge development at the transition state. Differences in the rates of hydrolysis between different series of hexopyranosides may not arise solely from field effects and likely also reflect differences in steric factors or solvation.
The use of the 2,4-dinitrophenyl group in sugar chemistry re-examined
Koeners, H. J.,Kok, A. J. de,Romers, C.,Boom, J. H. van
, p. 355 - 362 (2007/10/02)
The use of the tertiary base 1,4-diazabicyclooctane, together with 1-fluoro-2,4-dinitrobenzene in the solvent DMF, proved to be an efficient method for the introduction of the 2,4-dinitrophenyl (DNP) group at different positions, including the anom
