259824-49-2

Usage

Uses

Used in Analytical Chemistry:
2,4-Dinitrophenylhydrazine-d3 is used as a derivatizing agent for the detection and quantification of carbonyl groups in proteins and other analytes. The application reason is that DNPH-d3 reacts with carbonyl groups to form stable hydrazones, which can be easily detected and quantified using various analytical techniques such as mass spectrometry, high-performance liquid chromatography (HPLC), and ultraviolet-visible (UV-Vis) spectroscopy.
Used in Protein Analysis:
In the field of protein analysis, 2,4-Dinitrophenylhydrazine-d3 is used as a labeling agent to identify and quantify carbonyl groups in proteins. The application reason is that the formation of hydrazones with carbonyl groups provides a means to study protein oxidation, which is an essential post-translational modification involved in various biological processes and disease mechanisms.
Used in Pharmaceutical Research:
2,4-Dinitrophenylhydrazine-d3 is also used in pharmaceutical research as a tool to investigate the role of carbonyl groups in drug molecules. The application reason is that DNPH-d3 can help identify and quantify carbonyl-containing drugs, which can be crucial for understanding their chemical properties, stability, and potential interactions with biological targets.
Used in Environmental Science:
In environmental science, 2,4-Dinitrophenylhydrazine-d3 is employed as an analytical tool for the detection and quantification of carbonyl compounds in environmental samples. The application reason is that these carbonyl compounds can be important indicators of air and water pollution, and their accurate measurement is essential for assessing environmental quality and potential health risks.

Upstream product

• 3114-55-4 chlorobenzene-d⁽⁵⁾ 

Downstream Products

• 259824-62-9 C₉H₅⁽²⁾H₃N₄O₄ 

Relevant academic research and scientific papers

Application of screening experimental designs to assess chromatographic isotope effect upon isotope-coded derivatization for quantitative liquid chromatography-mass spectrometry

Isotope effect may cause partial chromatographic separation of labeled (heavy) and unlabeled (light) isotopologue pairs. Together with a simultaneous matrix effect, this could lead to unacceptable accuracy in quantitative liquid chromatography-mass spectrometry assays, especially when electrospray ionization is used. Four biologically relevant reactive aldehydes (acrolein, malondialdehyde, 4-hydroxy-2-nonenal, and 4-oxo-2-nonenal) were derivatized with light or heavy (d3-, 13C6-, 15N2-, or 15N4-labeled) 2,4-dinitrophenylhydrazine and used as model compounds to evaluate chromatographic isotope effects. For comprehensive assessment of retention time differences between light/heavy pairs under various gradient reversed-phase liquid chromatography conditions, major chromatographic parameters (stationary phase, mobile phase pH, temperature, organic solvent, and gradient slope) and different isotope labelings were addressed by multiple-factor screening using experimental designs that included both asymmetrical (Addelman) and Plackett-Burman schemes followed by statistical evaluations. Results confirmed that the most effective approach to avoid chromatographic isotope effect is the use of 15N or 13C labeling instead of deuterium labeling, while chromatographic parameters had no general influence. Comparison of the alternate isotope-coded derivatization assay (AIDA) using deuterium versus 15N labeling gave unacceptable differences (>15%) upon quantifying some of the model aldehydes from biological matrixes. On the basis of our results, we recommend the modification of the AIDA protocol by replacing d 3-2,4-dinitrophenylhydrazine with 15N- or 13C-labeled derivatizing reagent to avoid possible unfavorable consequences of chromatographic isotope effects.