26067-48-1Relevant academic research and scientific papers
Quantitative kinetic analysis in a microfluidic device using frequency-domain fluorescence lifetime imaging
Matthews, Sinead M.,Elder, Alan D.,Yunus, Kamran,Kaminski, Clemens F.,Brennan, Colin M.,Fisher, Adrian C.
, p. 4101 - 4109 (2007)
A novel microfluidic approach for the quantification of reaction kinetics is presented. A three-dimensional finite difference numerical simulation was developed in order to extract quantitative kinetic information from fluorescence lifetime imaging experimental data. This approach was first utilized for the study of a fluorescence quenching reaction within a microchannel; the lifetime of a fluorophore was used to map the diffusion of a quencher across the microchannel. The approach was then applied to a more complex chemical reaction between a fluorescent amine and an acid chloride, via numerical simulation the bimolecular rate constant for this reaction was obtained.
