26468-13-3Relevant academic research and scientific papers
Synthesis of chloro-substituted analogs of Thiazole orange - Fluorophores for flow cytometric analyses
Kaloyanova,Ivanova,Tchorbanov,Dimitrova,Deligeorgiev
experimental part, p. 215 - 221 (2012/03/11)
Synthesis, absorption and fluorescence properties of a series of asymmetrical monomethine cyanine dyes, chloro-containing analogs of Thiazole orange, are reported. Their staining ability was studied by flow cytometry. The saturating concentrations of each dye that gives a stable staining intensity have been determined. The ability of dyes B9, B11, B13 to stain live macrophages and apoptotic splenocytes was investigated. Positive signal in nucleus of adherent macrophages detected by fluorescent microscopy showed good specificity of B9, B11 and B13 dyes for DNA. In apoptotic assay cells positive for Annexin V were stained more brightly with the dyes B9, B11 and B13 than with propidium iodide. Despite that B13 showed high DNA selectivity it induces apoptosis of splenocytes and it is not suitable for detection of dead cells. The other synthesized chloro-containing analogs of Thiazole orange B9 and B11 can be successfully used for flow cytometric analyses of DNA content in live cells and for analyses of cell apoptosis.
Synthesis and fluorescence characteristics of novel asymmetric cyanine dyes for DNA detection
Kaloyanova, Stefka,Trusova, Valeriya M.,Gorbenko, Galyna P.,Deligeorgiev, Todor
scheme or table, p. 147 - 156 (2011/10/08)
Sixteen new asymmetric monomethine cyanine dyes have been synthesized and their spectral characteristics and interaction with double stranded DNA have been investigated. The dyes absorb in the region 453-519 nm and have molar absorptivities in the range 3
Heteroaromatic compounds having two-photon absorption activity
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, (2008/06/13)
The present invention relates to new heteroaromatic compounds having two-photon absorption activity. According to the invention, said compounds are suitable for use as optical power limiting agents via two-photon absorption or for use as imaging agents with two-photon absorbing activity for application in two-photon laser scanning confocal fluorescence microscopy. Compositions including said compounds and intermediates for their preparations are also within the scope of the present invention.
