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  • Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one,3',6'-dihydroxy-5(or 6)-isothiocyanato-/ LIDE PHARMA- Factory supply / Best price

    Cas No: 27072-45-3

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27072-45-3 Usage


Fluorescein isothiocyanate (FITC) is an amine-reactive derivative of fluorescein dye, characterized by its dark yellow powder form and hygroscopic nature. It is soluble in water and alkaline aqueous solutions, with an absorption maximum at 495 nm and an emission maximum at 525 nm. FITC is known for its strong green fluorescence, which disappears and precipitates when dissolved in an alkaline solution but reappears upon the addition of alkali. It is widely used as a label for antibodies and other probes in various applications, including fluorescence microscopy, flow cytometry, and immunofluorescence-based assays such as Western blotting and ELISA.


1. Used in Multifunctional Uniform Nanoparticles:
Fluorescein isothiocyanate is used as a fluorescent reagent in the development of multifunctional uniform nanoparticles composed of a magnetite nanocrystal core and a mesoporous silica shell. These nanoparticles are utilized for magnetic resonance, fluorescence imaging, and drug delivery applications.
2. Used in Fluorescent Labeling of Proteins:
FITC is employed as a fluorescent label for proteins, such as antibodies and lectins, in immunofluorescence applications. The isothiocyanate group reacts with amino terminal and primary amines in proteins, allowing for the attachment of a fluorescent label.
3. Used in Gel Chromatography:
Fluorescein isothiocyanate is used for protein labeling in gel chromatography, enabling the detection and analysis of proteins during the separation process.
4. Used as an Immunofluorescent Probe:
FITC is used to label antibodies for use as immunofluorescent probes, which are essential tools in the study of cellular and molecular processes.
5. Used in Hybridization Probes:
Fluorescein isothiocyanate is used to label oligonucleotides for hybridization probes, allowing for the detection and analysis of specific nucleic acid sequences.
6. Used in Detecting Proteins in Gels and on Western Blots:
FITC is utilized for detecting proteins in gels and on Western blots, providing a visual representation of protein expression and interactions.
7. Used as a Contact Sensitizer:
Fluorescein isothiocyanate isomer I has been proposed as a contact sensitizer, which may have potential applications in the study of allergic reactions and skin sensitization.


By 4-nitrophthalic anhydride and resorcinol at 195-200 ℃ for 10h, cyclized into a mixture of 3'-nitrofluorescein and 4'-nitrofluorescein, and then acetylated with acetic anhydride , add saturated sodium hydroxide-ethanol solution for hydrolysis to obtain 4'-nitrofluorescein. Then, 4'-nitrofluorescein was dissolved in ammonia water, and Nickel Reishi and hydrazine hydrate were added to react at room temperature for 2 hours, and then reduced to 4'-aminofluorescein. The most elevated by acylation, elimination of fluorescein isothiocyanate.

Check Digit Verification of cas no

The CAS Registry Mumber 27072-45-3 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 2,7,0,7 and 2 respectively; the second part has 2 digits, 4 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 27072-45:
103 % 10 = 3
So 27072-45-3 is a valid CAS Registry Number.

27072-45-3 Well-known Company Product Price

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  • TCI America

  • (F0784)  Fluorescein Isothiocyanate (mixture of 5- and 6- isomers)  >97.0%(HPLC)(T)

  • 27072-45-3

  • 100mg

  • 540.00CNY

  • Detail
  • TCI America

  • (F0784)  Fluorescein Isothiocyanate (mixture of 5- and 6- isomers)  >97.0%(HPLC)(T)

  • 27072-45-3

  • 1g

  • 3,100.00CNY

  • Detail
  • Sigma

  • (46950)  Fluorescein 5(6)-isothiocyanate  BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)

  • 27072-45-3

  • 46950-50MG-F

  • 305.37CNY

  • Detail
  • Sigma

  • (46950)  Fluorescein 5(6)-isothiocyanate  BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)

  • 27072-45-3

  • 46950-250MG-F

  • 993.33CNY

  • Detail
  • Sigma

  • (46950)  Fluorescein 5(6)-isothiocyanate  BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)

  • 27072-45-3

  • 46950-1G-F

  • 3,013.92CNY

  • Detail



According to Globally Harmonized System of Classification and Labelling of Chemicals (GHS) - Sixth revised edition

Version: 1.0

Creation Date: Aug 11, 2017

Revision Date: Aug 11, 2017


1.1 GHS Product identifier

Product name Fluorescein isothiocyanate

1.2 Other means of identification

Product number -
Other names FITC-I

1.3 Recommended use of the chemical and restrictions on use

Identified uses For industry use only.
Uses advised against no data available

1.4 Supplier's details

1.5 Emergency phone number

Emergency phone number -
Service hours Monday to Friday, 9am-5pm (Standard time zone: UTC/GMT +8 hours).

More Details:27072-45-3 SDS

27072-45-3Relevant articles and documents

Preparation method of fluorescein probe with specific selectivity


, (2021/05/29)

The invention relates to the field of preparation of fluorescein probes with specific selectivity, and particularly discloses a preparation method of a fluorescein probe with specific selectivity. The method comprises the following steps: (1) mixing 4-nitrophthalic anhydride, resorcinol, nitrocyclohexane and anhydrous cobalt chloride, conducting heating and melting, adding zinc chloride, conducting reacting, then adding hydrochloric acid for reflux, and conducting filtering, washing and drying; (2) mixing a reducing solution containing Na2S and Na2SO3 with a product obtained in the step (1), conducting reacting, then dropwise adding glacial acetic acid until complete precipitation, and conducting filtering, washing and drying; and (3) mixing a mixed solvent containing methanol and triethylamine with a product obtained in the step (2), dropwise adding CS2, conducting reacting, dropwise adding a copper chloride aqueous solution, continuing reacting after dropwise adding, adjusting the pH value of the system to 1-2 by using hydrochloric acid, and carrying out reduced pressure distillation, washing, drying and column chromatography purification. According to the method, the conversion rate and the yield of the 5-fluorescein isothiocyanate are greatly improved.

Method for preparing 5-fluorescein isothiocyanate ester


Paragraph 0044; 0045; 0046; 0047, (2017/07/19)

The invention provides a method for preparing 5-fluorescein isothiocyanate ester. The method comprises the following steps: (1) reacting an original material 5-amino fluorescein with carbon disulfide in the presence of a catalyst and a solvent, so as to obtain fluorescein-5-amine disulfide formate; and (2) reacting fluorescein-5-amine disulfide formate, obtained in the step (1), in the presence of the catalyst and the solvent, so as to obtain 5-fluorescein isothiocyanate ester. The method has the beneficial effects that the technical operational safety is relatively high, the production cost is low, a synthetic process is simple, and the reaction condition is mild; and 5-fluorescein isothiocyanate ester is high in yield and wide in application range and can adequately meet the requirements of industrial production of products.

Fluorescent human EP3 receptor antagonists

Tomasch, Miriam,Schwed, J. Stephan,Kuczka, Karina,Meyer Dos Santos, Sascha,Harder, Sebastian,Nuesing, Rolf M.,Paulke, Alexander,Stark, Holger

supporting information, p. 774 - 779 (2012/11/13)

Exchange of the lipophilc part of ortho-substituted cinnamic acid lead structures with different small molecule fluorophoric moieties via a dimethylene spacer resulted in hEP3R ligands with affinities in the nanomolar concentration range. Synthesized compounds emit fluorescence in the blue, green, and red range of light and have been tested concerning their potential as a pharmacological tool. hEP3Rs were visualized by confocal laser scanning microscopy on HT-29 cells, on murine kidney tissues, and on human brain tissues and functionally were characterized as antagonists on human platelets. Inhibition of PGE2 and collagen-induced platelet aggregation was measured after preincubation with novel hEP3R ligands. The pyryllium-labeled ligand 8 has been shown as one of the most promising structures, displaying a useful fluorescence and highly affine hEP3R antagonists.

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