28643-80-3 Usage
Description
Nigericin Na (28643-80-3) is a polyether ionophore which disrupts membrane potential and stimulates ATPase activity in mitochondria. Induces apoptosis resulting from intracellular acidification. Ion selectivity for nigericin Na is K+> Rb+> Cs+>>Na+. Cell permeable.
Chemical Properties
NIGERICIN SODIUM SALT is white powder
Uses
Different sources of media describe the Uses of 28643-80-3 differently. You can refer to the following data:
1. Nigericin sodium is the atypical salt of nigericin. Since nigericin is an ionophore, its very high affinity for monovalent cations such as Na+ and K+ means that formation of a salt is a facile possess occurring during purification under any but highly acidic conditions. Typically, the salts of polyether ionophores like the free acid, are readily extracted into organic solvents. The sodium ion is stabilised within a polar pocket of the structure, effectively making the salt and free acid different chemical moieties with the potential for differing pharmacology, a fact not readily appreciated in the literature.
2. Nigericin sodium is a salt of the atypical polyether antibiotic, nigericin. Since nigericin is an ionophore, its very high affinity for monovalent cations such as Na+ and K+ means that formation of a salt is a facile possess occurring during purification under any but highly acidic conditions. Typically, the salts of polyether ionophores like the free acid, are readily extracted into organic solvents. The sodium ion is stabilised within a polar pocket of the structure, effectively making the salt and free acid different chemical moieties with the potential for differing pharmacology, a fact not readily appreciated in the literature.
3. Nigericin sodium salt is a polyether ionophore which catalyzes the electroneutral exchange of alkali metal (K+) for H+ (antiport). It disrupts membrane potential and stimulates ATPase activity in mitochondria. It transports monovalent cations across membranes with the following specificity: K+ >Rb+ >Cs+ >>Na. It kills bacteria by facilitating the diffusion of ions across membranes. It is widely used in studies of the consequences of changes in membrane potential in variable systems.
General Description
A monocarboxylate that catalyzes the electroneutral exchange of an alkali metal for H+ (antiport). Transports monovalent cations across membranes with the following specificities: K+>Rb+>Cs+>>Na+. An antibiotic that kills bacteria by facilitating the diffusion of ions across membranes. Disrupts the membrane potential and stimulates mitochondrial ATPase activity.
Biochem/physiol Actions
Product does not compete with ATP.
References
1) Akhmedov et al. (2010), Mitochondrial matrix pH controls oxidative phosphorylation and metabolism-secretion coupling in INS-1E clonal beta cells; FASEB J., 24 4613
2) Gong et al. (2010), Chemical probing reveals insights into the signaling mechanism of inflammasome activation; Cell Res., 20 1289
Check Digit Verification of cas no
The CAS Registry Mumber 28643-80-3 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 2,8,6,4 and 3 respectively; the second part has 2 digits, 8 and 0 respectively.
Calculate Digit Verification of CAS Registry Number 28643-80:
(7*2)+(6*8)+(5*6)+(4*4)+(3*3)+(2*8)+(1*0)=133
133 % 10 = 3
So 28643-80-3 is a valid CAS Registry Number.
InChI:InChI=1/C40H68O11.Na/c1-21-11-12-28(46-33(21)26(6)36(42)43)17-29-18-30(45-10)27(7)40(48-29)25(5)19-38(9,51-40)32-13-14-37(8,49-32)35-23(3)16-31(47-35)34-22(2)15-24(4)39(44,20-41)50-34;/h21-35,41,44H,11-20H2,1-10H3,(H,42,43);/q;+1/p-1/t21-,22-,23-,24+,25?,26?,27-,28+,29+,30+,31+,32?,33?,34-,35?,37?,38?,39-,40+;/m0./s1
28643-80-3Relevant articles and documents
ETUDE THERMODYNAMIQUE DE LA COMPLEXATION DES IONS SODIUM, POTASSIUM ET RUBIDIUM PAR LES IONOPHORES BACTERIENS: MONENSINE, GRISORIXINE ET NIGERICINE
Pointud, Yvon,Juillard, Jean,Jeminet, Georges,David, Lucien
, p. 67 - 72 (2007/10/02)
Enthalpies in anhydrous methanol for both dissociation of carboxylic group and complexation of alkali metal cations by these natural ionophores (as 1-1 neutral association between the cation and the carboxylate form of the ionophore) are obtained from calorimetric measurements.Using equilibrium constants measured independently, it is possible to reach the corresponding entropies.The results now obtained on the thermodynamic of these two reactions are discussed in terms of "opened" or "closed" structure of the ionophores and of interactions with the solvent medium: - both acid and "empty" anion form can be considered as open in methanol; - nigericin and grisorixin have, as far as G, H, S, are concerned a very similar behaviour.The standard quantities are only shifted from grisorixin to nigericin, owing to the solvation of the supplementary OH group of the second ionophore; - monensin have a very distinct behaviour from the two other ionophores.Such thermodynamic studies are expected to give some informations on the mechanism of action of these bacterial ionophores.
