2906-42-5Relevant academic research and scientific papers
Biosynthesis of trigonelline from nicotinate mononucleotide in mungbean seedlings
Zheng, Xin-Qiang,Matsui, Ayu,Ashihara, Hiroshi
, p. 390 - 395 (2008)
To determine the biosynthetic pathway to trigonelline, the metabolism of [carboxyl-14C]nicotinate mononucleotide (NaMN) and [carboxyl-14C]nicotinate riboside (NaR) in protein extracts and tissues of embryonic axes from germinating mungbeans (Phaseolus aureus) was investigated. In crude cell-free protein extracts, in the presence of S-adenosyl-l-methionine, radioactivity from [14C]NaMN was incorporated into NaR, nicotinate and trigonelline. Activities of NaMN nucleotidase, NaR nucleosidase and trigonelline synthase were also observed in the extracts. Exogenously supplied [14C]NaR, taken up by embryonic axes segments, was readily converted to nicotinate and trigonelline. It is concluded that the NaMN → NaR → nicotinate → trigonelline pathway is operative in the embryonic axes of mungbean seedlings. This result suggests that trigonelline is synthesised not only from NAD but also via the de novo biosynthetic pathway of pyridine nucleotides.
Synthesis of three alpha 7 agonists in labeled form
Elmore, Charles S.,Landvatter, Scott,Dorff, Peter N.,Powell, Mark E.,Killick, David,Blake, Timothy,Hall, James,Heys, J. Richard,Harding, John,Urbanek, Rebecca,Ernst, Glen
, p. 342 - 349 (2014/06/10)
In support of a program to develop an alpha 7 agonist as a treatment for Alzheimer's disease, three drug candidates, 1, 2, and 3, were prepared in labeled forms. Compound 1 was prepared in C-14 labeled form by lithiation of [2,6-14C2]2-chloropyridine and subsequent coupling with spirooxirane-2,3'-quinuclidine. When this same coupling was attempted using [3,4,5,6-2H4]2-chloropyridine, alcohol [2H 6]-6 was the major product indicating that the primary isotope effect for the lithiation step was significant enough to shift the reaction pathway. Therefore, an alternate site of labeling was used to prepare [2H 4]-1. [13C5]-2 was prepared in five steps from [13C5]2-furoic acid, but the C-14 labeled compound used [14C2]-1 as the starting material instead. [ 14C2]-3 was prepared in two steps from [carbonyl- 14C]nicotinic acid. Copyright
