299931-15-0Relevant academic research and scientific papers
One-step synthesis of biotinyl photoprobes from unprotected carbohydrates
Hatanaka, Yasumaru,Kempin, Uwe,Jong-Jip, Park
, p. 5639 - 5643 (2000)
A simple and versatile approach for the preparation of carbohydrate photoprobes has been developed. By a single-step reaction at 37 °C, a biotinylated carbene-generating unit was introduced to the reducing end of unprotected carbohydrates. Micromole quantities of N-acetyllactosamine, Lewis X trisaccharide, and sialyl Lewis X tetrasaccharide were easily converted to their biotinylated photoreactive analogues, which enabled the nonradioisotopic chemiluminescent detection of the photolabeled products. Thus, a sequence of lectin photoaffinity labeling, from the probe synthesis to the detection of labeled protein, was readily accomplished within one week. Our strategy may be applicable to any aldehyde-bearing ligand.
Phenyldiazirine compounds and photoaffinity labeling reagent
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Page/Page column 13-14, (2010/02/11)
This invention offers a photoreactive labeling reagent consisting of a novel biotinylated phenyldiazirine compound; a photoaffinity labeling reagent consisting of a saccharide-linked biotinylated phenyldiazirine compound where a saccharide compound is int
