301662-92-0

Upstream product

• 173029-44-2 phenyl (3,4-di-O-isopropylidene-β-D-galactopyranosyl)-(1->4)-1-thio-β-D-glucopyranoside 
• 98-88-4 benzoyl chloride 
• 301662-87-3 phenyl 2,6-di-O-benzoyl-3,4-di-O-isopropylidene-β-D-galactopyranosyl-(1->4)-2,3,6-tri-O-benzoyl-1-thio-β-D-glucopyranoside 

Downstream Products

• 301663-04-7 phenyl 3,4,6-tri-O-acetyl-2-deoxy-2-phthalimido-β-D-glucopyranosyl-(1 > 3)-2,6-di-O-benzoyl-β-D-galactopyranosyl-(1-> 4)-2,3,6-tri-O-benzoyl-1-thio-β-D-glucopyranoside 

Relevant academic research and scientific papers

Polymer-supported and chemoenzymatic synthesis of the Neisseria meningitidis pentasaccharide: A methodological comparison

Neisseria meningitids trisaccharide [GlcNAcβ(1 → 3)Galβ(1 → 4)Glc-R], tetrasaccharide [Galβ(1 → 4)GlcNAcβ(1 → 3)Galβ(1 → 4)Glc-R], and a pentasaccharide [Neu5Acα(2 → 3)Galβ(1 → 4)GlcNAcβ(1 → 3)Galβ(1 → 4)Glc-SPh] were prepared via conventional chemical synthesis, polymer-supported synthesis, and chemoenzymatic methods, starting from D-lactose. The polymer polyethyleneglycol monomethylether (MPEG) and the linker dioxyxylene (DOX) were used with a lactose-bound acceptor to improve the purification process. Several enzymes (LgtA, GalE-LgtB fusion, and CMP-Neu5Ac synthetase/sialyltransferase fusion) were used for syntheses of these oligosaccharides. Excellent stereo- and regioselectivities as well high yield (> 90% from Galβ(1 → 4)Glc-SPh) of the pentasaccharide were obtained. Both of the convenient processes are suitable for efficient preparation of target oligosaccharides. (C) 2000 Elsevier Science Ltd.