31528-00-4Relevant articles and documents
Improved enantioselectivity of thermostable esterase ST0071 from archaeon Sulfolobus tokodaii by site-saturation mutagenesis
Ozaki, Masanaru,Kawakami, Norifumi,Ohta, Hiromichi,Miyamoto, Kenji
, p. 249 - 252 (2016)
An archaeon GGG(A)X-type esterase (ST0071) can catalyze the hydrolysis of various acetates of secondary alcohols, but shows low enantioselectivity. Using structure-guided site-saturation mutagenesis, we successfully identified a G274W variant that has excellent selectivity compared with that of wild-type ST0071.