3326-32-7

Usage

Uses

1. Used in Biochemical Applications:
Fluorescein isothiocyanate isomer I is used as a fluorescent marker for the modification of actin at lys-61, labeling of FAB, and the modification of thiol groups.
2. Used in Microbiology:
Fluorescein isothiocyanate isomer I is used as a staining agent for the staining of conidia.
3. Used in Immuno Histology/Cytology:
Fluorescein isothiocyanate isomer I is used as a fluorescent labeling reagent for proteins and for rapid identification of pathogens in the fluorescent antibody technique.
4. Used in Drug Delivery Systems:
Fluorescein isothiocyanate isomer I is used as a reagent for microsequencing of proteins and peptides (HPLC) and for the labeling of fibrinogen on celite.
5. Used in Clinical Diagnostics:
Fluorescein isothiocyanate isomer I is used in immuno histology/cytology for the visualization and detection of tissue-bound biomolecules like antigens, lectins, various proteins, peptides, nucleic acids, oligoand polysaccharides in samples of human origin. It is an IVD registered product and CE certified, making it suitable for clinical diagnostic purposes.
6. Used in Research and Development:
Fluorescein isothiocyanate isomer I is used as a fluorescent probe capable of being conjugated to tissue and proteins, which is useful in various research and development applications involving the study of biomolecular interactions and detection.

Purification Methods

It is made from the pure 5-amino isomer. Purify it by dissolving it in boiling Me2CO, filtering and adding pet ether (b 60-70o) until it becomes turbid. If an oil separates, then decant it and add more pet ether to the supernatant and cool. Orange-yellow crystals separate, collect and dry them in vacuo. It should give one spot on TLC (silica gel) in EtOAc/pyridine/AcOH (50:1:1) and in Me2NCHO/CHCl3/28% NH4OH (10:5:4). IR (Me2SO): 2110 (NCS) and 1760 (C=O) cm-1. The max 1HNMR spectra in Me2CO-d6 of the 5-and 6-isomers are distinctly different for the protons in the *benzene ring; the UV in phosphate buffer pH 8.0 shows a at ~490nm. [Sinsheimer et al. Anal Biochem 57 2271974, McKinney et al. Anal Biochem 7 74 1964, Beilstein 19 III/IV 4337.]

InChI:InChI=1/C21H11NO5S/c23-12-2-5-15-18(8-12)27-19-9-13(24)3-6-16(19)20(15)14-4-1-11(22-10-28)7-17(14)21(25)26/h1-9,23H,(H,25,26)

Upstream product

• 3326-35-0 3',6'-dihydroxy-5-nitrospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one 
• 610-27-5 4-nitrophthalic acid 
• 108-46-3 recorcinol 
• 14926-29-5 3',6'-bis(acetyloxy)-5-nitrospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one 
• 463-71-8 thiophosgene 
• 3326-34-9 fluoresceinamine 
• 1122-60-7 1-nitrocyclohexane 
• 5466-84-2 4-nitrophthalic anhydride 
• 75-15-0 carbon disulfide 
• 2321-07-5 fluorescein 

Downstream Products

• 75453-82-6 1-(2-aminoethyl)-3-(3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5-yl)thiourea 
• 228850-32-6 8-{carboxy-[2-(fluoresceinylthioureido)ethyl]amido}octyl α-D-mannopyranoside 

Relevant academic research and scientific papers

Preparation method of fluorescein probe with specific selectivity

The invention relates to the field of preparation of fluorescein probes with specific selectivity, and particularly discloses a preparation method of a fluorescein probe with specific selectivity. The method comprises the following steps: (1) mixing 4-nitrophthalic anhydride, resorcinol, nitrocyclohexane and anhydrous cobalt chloride, conducting heating and melting, adding zinc chloride, conducting reacting, then adding hydrochloric acid for reflux, and conducting filtering, washing and drying; (2) mixing a reducing solution containing Na2S and Na2SO3 with a product obtained in the step (1), conducting reacting, then dropwise adding glacial acetic acid until complete precipitation, and conducting filtering, washing and drying; and (3) mixing a mixed solvent containing methanol and triethylamine with a product obtained in the step (2), dropwise adding CS2, conducting reacting, dropwise adding a copper chloride aqueous solution, continuing reacting after dropwise adding, adjusting the pH value of the system to 1-2 by using hydrochloric acid, and carrying out reduced pressure distillation, washing, drying and column chromatography purification. According to the method, the conversion rate and the yield of the 5-fluorescein isothiocyanate are greatly improved.

REAGENT KIT USED FOR DETECTING GASTRIN-17, AND PREPARATION METHOD AND APPLICATION FOR REAGENT KIT

A reagent kit used for detecting gastrin-17, a preparation method, and a detection method for the reagent kit. The reagent kit comprises component A and component B, wherein component A is a first anti-gastrin-17 antibody marked with a trace marker or coated on magnetic spheres, and component B is a second gastrin-17 antibody or gastrin-17 coated on magnetic spheres or marked with a trace marker. Either one of component A and component B is marked with the trace marker, and the other one is coated on the magnetic spheres. The first gastrin-17 antibody and gastrin-17 binding site is different from the second gastrin-17 antibody and gastrin-17 binding site. The method using a double antibody sandwich method or a competition method to detect gastrin-17 accurately and sensitively measures the amount of gastrin-17 in a sample.

Method for preparing 5-fluorescein isothiocyanate ester

The invention provides a method for preparing 5-fluorescein isothiocyanate ester. The method comprises the following steps: (1) reacting an original material 5-amino fluorescein with carbon disulfide in the presence of a catalyst and a solvent, so as to obtain fluorescein-5-amine disulfide formate; and (2) reacting fluorescein-5-amine disulfide formate, obtained in the step (1), in the presence of the catalyst and the solvent, so as to obtain 5-fluorescein isothiocyanate ester. The method has the beneficial effects that the technical operational safety is relatively high, the production cost is low, a synthetic process is simple, and the reaction condition is mild; and 5-fluorescein isothiocyanate ester is high in yield and wide in application range and can adequately meet the requirements of industrial production of products.

Single label comparative hybridization

The present invention provides methods of detecting and mapping chromosomal or genetic abnormalities associated with various diseases or with predisposition to various diseases, or to detecting the phenomena of large scale copy number variants. In particular, the present invention provides advanced methods of performing array-based comparative hybridization that allow reproducibility between samples and enhanced sensitivity by using the same detectable label for both test sample and reference sample nucleic acids. Invention methods are useful for the detection or diagnosis of particular disease conditions such as cancer, and detecting predisposition to cancer based on detection of chromosomal or genetic abnormalities and gene expression level. Invention methods are also useful for the detection or diagnosis of hereditary genetic disorders or predisposition thereto, especially in prenatal samples. Moreover, invention methods are also useful for the detection or diagnosis of de novo genetic aberrations associated with post-natal developmental abnormalities.

Fluorescent human EP3 receptor antagonists

Exchange of the lipophilc part of ortho-substituted cinnamic acid lead structures with different small molecule fluorophoric moieties via a dimethylene spacer resulted in hEP3R ligands with affinities in the nanomolar concentration range. Synthesized compounds emit fluorescence in the blue, green, and red range of light and have been tested concerning their potential as a pharmacological tool. hEP3Rs were visualized by confocal laser scanning microscopy on HT-29 cells, on murine kidney tissues, and on human brain tissues and functionally were characterized as antagonists on human platelets. Inhibition of PGE2 and collagen-induced platelet aggregation was measured after preincubation with novel hEP3R ligands. The pyryllium-labeled ligand 8 has been shown as one of the most promising structures, displaying a useful fluorescence and highly affine hEP3R antagonists.

LAYERED NANOPARTICLES

The invention provides a process for forming a layered nanoparticle, comprising providing a suspension comprising a core particle in a first liquid, adding a second liquid to the suspension, and adding a reagent, or a precursor for the reagent, to the suspension. The second liquid is immiscible with the first liquid. If the reagent is added to the suspension, the reagent reacts to form a layer on the core particle to form the layered nanoparticle. If a precursor for the reagent is added to the suspension, the precursor is converted to the reagent, and the reagent reacts to form a layer on the core particle to form the layered nanoparticle.

Method for locating hidden microorganism contaminated surfaces in industrial water systems

A method for locating hidden microorganism contaminated surfaces in industrial water systems is described and claimed. The method works by applying a solution or dispersion of a fluorogenic reagent in water to the hidden water contact surfaces of said industrial water system and allowing said fluorogenic reagent to react with any hidden microorganisms present, wherein said fluorogenic reagent is selected from the group of fluorogenic reagents that are known to react with microorganisms such that a fluorescent signal of said fluorogenic reagent is detectable in such a way as to make the detection of the fluorescent signal indicate that there are hidden microorganisms present on the water contact surfaces of the equipment and piping.