335595-78-3

Upstream product

• 18162-48-6 tert-butyldimethylsilyl chloride 
• 335595-73-8 [1'-13C]-3',5'-O-(di-tert-butylsilanediyl)-N⁴-benzoylcytidine 
• 26661-13-2 4-N-benzoylcytosine 
• 60599-10-2 N⁴-benzoyl-bis(trimethylsilyl)cytosine 
• 335595-68-1 [1'-13C]-N⁴-benzoylcytidine 
• 144963-48-4 [1'-13C]-2',3',5'-tri-O-benzoyl-N⁴-benzoylcytidine 

Downstream Products

• 335595-81-8 [1'-13C]-2'-O-tert-butyldimethylsilyl-N⁴-benzoylcytidine 

Relevant academic research and scientific papers

Synthesis of isotopically labeled D-[1′-13C]ribonucleoside phosphoramidites

The preparation of fully protected labeled diisopropylamino-β-cyanoethyl-[1′-13C]ribonucleoside phosphoramidites with regioisomeric purity is described. We demonstrated in this paper that a regioselective 2′-O-silylation, through a 3′,5′-O-di-tert-butylsilanediyl protection, has been applied for the synthesis of [1′-13C]ribonucleoside phosphoramidite units. This method allowed us to obtain only the desired 2′-O-silyl-3′-O-phosphoramidites avoiding the undesired 3′-O-silyl-2′-O-phosphoramidite nucleosides isolated by standard procedures. This is a suitable procedure to RNA precursors with respect to the isotope-containing precursors.