33608-52-5Relevant articles and documents
Synthesis of a new fluorogenic substrate for the assay of phosphoinositide-specific phospholipase C.
Rukavishnikov, Aleksey V.,Smith, Miles P.,Birrell, G. Bruce,Keana, John F.W.,Griffith, O. Hayes
, p. 6637 - 6640 (1998)
The synthesis of a fluorescein-derived fluorogenic substrate, D,L-myo- inositol 1-([6'-hexyloxy-3-oxo]spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'- yl hydrogen phosphate) sodium salt 9, for assay of activity of phosphatidylinositide-specific phospholipase C (PI-PLC), is described. This substrate is shown to be a sensitive substrate for bacterial PI-PLC in a continuous, non-radioactive assay.
