342630-70-0

Basic information

• Product Name: 5'-O-(4,4'-Dimethoxytrityl)-2-isobutyramido-N⁶-(1-oxyl-2,2,6,6-tetramethyl-4-piperidinyl)-2'-deoxyadenosine
• Synonyms: 5'-O-(4,4'-Dimethoxytrityl)-2-isobutyramido-N⁶-(1-oxyl-2,2,6,6-tetramethyl-4-piperidinyl)-2'-deoxyadenosine
• CAS NO: 342630-70-0
• Molecular Weight: 794.971
• Mol File: 342630-70-0.mol

Chemical Properties

• CAS DataBase Reference: 5'-O-(4,4'-Dimethoxytrityl)-2-isobutyramido-N⁶-(1-oxyl-2,2,6,6-tetramethyl-4-piperidinyl)-2'-deoxyadenosine(CAS DataBase Reference)
• NIST Chemistry Reference: 5'-O-(4,4'-Dimethoxytrityl)-2-isobutyramido-N⁶-(1-oxyl-2,2,6,6-tetramethyl-4-piperidinyl)-2'-deoxyadenosine(342630-70-0)
• EPA Substance Registry System: 5'-O-(4,4'-Dimethoxytrityl)-2-isobutyramido-N⁶-(1-oxyl-2,2,6,6-tetramethyl-4-piperidinyl)-2'-deoxyadenosine(342630-70-0)

Safety Data

• Hazardous Substances Data: 342630-70-0(Hazardous Substances Data)

Upstream product

• 40615-36-9 4,4'-dimethoxytrityl chloride 
• 342630-68-6 2-Isobutyramido-N⁶-(1-oxyl-2,2,6,6-tetramethyl-4-piperidinyl)-2'-deoxyadenosine 
• 82921-43-5 2-N-3',5'-O-triisobutyryl-6-O-(2,4,6-triisopropylbenzenesulfonyl)-2'-deoxyguanosine 
• 342630-66-4 3',5'-Di-O-isobutyryl-2-isobutyramido-N⁶-(1-oxyl-2,2,6,6-tetramethyl-4-piperidinyl)-2'-deoxyadenosine 
• 36768-62-4 4-AMINO-2,2,6,6-TETRAMETHYLPIPERIDINE 
• 14691-88-4 tempamine 
• 14691-89-5 4-acetylamino-2,2,6,6-tetramethylpiperidine-N-oxyl 
• 4168-79-0 4-hydroxyimino-2,2,6,6-tetramethylpiperidine 
• 342630-66-4 3',5'-diisobutyryl-2-isobutyramido-N₆-(1-oxyl-2,2,6,6-tetramethyl-4-piperidinyl)-2'-deoxyadenosine 
• 342630-68-6 3',5'-diisobutyryl-2-isobutyramido-N6-(1-oxyl-2,2,6,6-tetramethyl-4-piperidinyl)-2'-deoxyadenosine 
• 136708-43-5 4-acetylamino-2,2,6,6-tetramethyl-1-piperidinium acetate 
• 961-07-9 2'-Deoxyguanosine 
• 82921-42-4 2'-deoxy-N²-isobutyrylguanosine 3',5'-diisobutyrate 

Downstream Products

• 342630-72-2 5'-dimethoxytrityl-2-isobutyramido-N₆-(1-oxyl-2,2,6,6-tetramethyl-4-piperidinyl)-2'-deoxyadenosine-3'-(2-cyanoethyl-N,Ndiisopropyl) phosphoramidite 

Relevant articles and documents

Electron paramagnetic resonance (EPR) study of spin-labeled camptothecin derivatives: A different look of the ternary complex

Camptothecin (CPT) derivatives are clinically effective poisons of DNA topoisomerase I (Top1) able to form a ternary complex with the Top1-DNA complex. The aim of this investigation was to examine the dynamic aspects of the ternary complex formation by means of site-directed spin labeling electron paramagnetic resonance (SDSL-EPR). Two semisynthetic CPT derivatives bearing the paramagnetic moiety were synthesized, and their biological activity was tested. A 22-mer DNA oligonucleotide sequence with high affinity cleavage site for Top1 was also synthesized. EPR experiments were carried out on modified CPT in the presence of DNA, of Top1, or of both. In the last case, a slow motion component in the EPR signal appeared, indicating the formation of the ternary complex. Deconvolution of the EPR spectrum allowed to obtain the relative drug amounts in the complex. It was also possible to demonstrate that the residence time of CPT "trapped" in the ternary complex is longer than hundreds of microseconds.

Preparation of spin-labeled 2-amino-dA, dA, dC and 5-methyl-dC phosphoramidites for the automatic synthesis of EPR active oligonucleotides

2,2,6,6-Tetramethyl-1-piperidinyloxy free radical (TEMPO) labeled phosphoramidites of 2-amino-dA, dA, dC and 5-methyl-dC were synthesized and used for the automatic synthesis of mono-labeled oligodeoxynucleotides (ODNs), which proved active to EPR. It is now possibile to insert a paramagnetic probe into nucleic acids in a site- and type-specific manner. The combination of this synthetic approach with EPR spectroscopy can be exploited for performing studies on dynamics and local structural modifications in nucleic acids.