3520-43-2 Usage
Uses
Used in Biomedical Research:
JC-1 is used as a dual-emission potential-sensitive probe for measuring mitochondrial membrane potential. It is particularly useful in studying cellular apoptosis and mitochondrial integrity.
Used in Flow Cytometry:
JC-1 is used as a fluorescence marker for detecting changes in mitochondrial transmembrane potential (ΔΨm) in cells. It helps in distinguishing between healthy cells with high ΔΨm and apoptotic cells with low ΔΨm by fluorescing red/orange for J-aggregates and green for monomers, respectively.
Used in Fluorescent Microscopy:
JC-1 is used as a staining agent for visualizing mitochondrial changes in cells under a fluorescent microscope. It allows researchers to observe the transition from J-aggregates to monomers, indicating a decrease in mitochondrial transmembrane potential.
Used in Plate Readers:
JC-1 is used as a fluorescence indicator in plate readers for high-throughput screening of mitochondrial membrane potential in cell populations. It enables the quantification of ΔΨm changes in response to various treatments or conditions.
References
1) Reers et al. (1991), J-aggregate formation of a carbocyanine as a quantitative fluorescent indicator of membrane potential; Biochemistry, 30 4480
2) Smiley et al. (1991), Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1; Proc. Natl. Acad. Sci. USA, 88 3671
3) Lisa et al. (1995), Mitochondrial membrane potential in single living adult rat cardiac myocytes exposed to anoxia or metabolic inhibition; J. Physiol., 486 1
4) Wolosin et al. (2017), Application of JC1 for non-toxic isolation of cells with MDR transporter activity by flow cytometry; PLoS One, 12(4) e0174905
5) Wang et al. (2017), A novel cytoprotective peptide protects mesenchymal stem cells against mitochondrial dysfunction and apoptosis induced by starvation via Nrf2/Sirt3/FoxO3a pathway; J. Transl. Med., 15 33
Check Digit Verification of cas no
The CAS Registry Mumber 3520-43-2 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 3,5,2 and 0 respectively; the second part has 2 digits, 4 and 3 respectively.
Calculate Digit Verification of CAS Registry Number 3520-43:
(6*3)+(5*5)+(4*2)+(3*0)+(2*4)+(1*3)=62
62 % 10 = 2
So 3520-43-2 is a valid CAS Registry Number.
InChI:InChI=1/C25H27Cl4N4.HI/c1-5-30-20-12-16(26)17(27)13-21(20)31(6-2)24(30)10-9-11-25-32(7-3)22-14-18(28)19(29)15-23(22)33(25)8-4;/h9-15H,5-8H2,1-4H3;1H/q+1;/p-1
3520-43-2Relevant academic research and scientific papers
MACROPHAGE IDENTIFICATION AGENT, AND IDENTIFICATION METHOD, SORTING METHOD, EVALUATION METHOD, SCREENING METHOD AND KIT USING THE MACROPHAGE IDENTIFIER AGENT
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Paragraph 0363, (2016/02/28)
An object of the present invention is to provide an identification method for a macrophage subtype using an organic compound. Another object of the present invention is to provide a macrophage identification agent containing an organic compound in which a spectral characteristic obtained when the organic compound is added is different depending on a macrophage subtype. Still another object of the present invention is to provide an evaluation method for a macrophage subtype using a macrophage identification agent, an analysis method for correlation between a macrophage subtype and a test substance, a screening method for correlation between a macrophage subtype and a test substance, and a kit. An identification method for a macrophage subtype utilizing that a spectral characteristic obtained with an organic compound added is different depending on a macrophage subtype is provided.
The structure of benzimidazole cyanine dyes, their spectroscopy and their performance in photographic emulsions
Peng, Zheng-Hong,Geise,Zhou, Xiang-Feng,Peng, Bi-Xian,Carleer,Dommisse
, p. 27 - 33 (2007/10/03)
The multi-step synthesis of two benzimidazole trimethine cyanines as possible green sensitizing dyes (Gs3-1, Gs3-2) is reported. Compounds were characterized from UV-Vis, mass spectroscopic and NMR data. The charge density distributions of the two compounds, calculated using the semi-empirical MOPAC 93 package, are in agreement with assignments in NMR spectroscopy. Sensitizing properties of the cyanine dyes were evaluated in actual photographic T-grain emulsions. VCH Verlagsgescllschaft mbH, 1997.
