353455-91-1Relevant academic research and scientific papers
Laccase-catalysed oxidation of ferulic acid and ethyl ferulate in aqueous medium: A green procedure for the synthesis of new compounds
Aljawish, Abdulhadi,Chevalot, Isabelle,Jasniewski, Jordane,Paris, Cedric,Scher, Joel,Muniglia, Lionel
, p. 1046 - 1054 (2014)
The enzymatic oxidation of ferulic acid (FA) and ethyl ferulate (EF) with Myceliophthora thermophila laccase, as biocatalyst, was performed in aqueous medium using an eco-friendly procedure to synthesize new active molecules. First, the commercial laccase was ultrafiltrated allowing for the elimination of phenolic contaminants and increasing the specific activity by a factor of 2. Then, kinetic parameters of this laccase were determined for both substrates (FA, EF), indicating a higher substrate affinity for ethyl ferulate. Additionally, enzymatic oxidation led to the synthesis of a FA-major product, exhibiting a molecular mass of 386 g/mol and a EF-major product with a molecular mass of 442 g/mol. Structural analyses by mass spectrometry allowed the identification of dimeric derivatives. The optical properties of the oxidation products showed the increase of red and yellow colours, with FA-products compared to EF-products. Additionally, enzymatic oxidation led to a decrease of antioxidant and cytotoxic activities compared to initial substrates. Consequently, this enzymatic procedure in aqueous medium could provide new compounds presenting optical, antioxidant and cytotoxic interest.
Structural Transformation of 8-5-Coupled Dehydrodiferulates by Human Intestinal Microbiota
Schendel, Rachel R.,Karrer, Cecile,Bunzel, Diana,Huch, Melanie,Hildebrand, Andreas A.,Kulling, Sabine E.,Bunzel, Mirko
, p. 7975 - 7985 (2015)
Ingested dehydrodiferulates (DFAs) are partially released from cereal dietary fiber by human colonic microbiota, but little research has explored the further microbial metabolism of 8-5-coupled DFAs. This study investigated the in vitro microbial metaboli
Laccase-catalyzed dimerization of ferulic acid amplifies antioxidant activity
Adelakun, Oluyemisi E.,Kudanga, Tukayi,Parker, Ayesha,Green, Ivan R.,Le Roes-Hill, Marilize,Burton, Stephanie G.
experimental part, p. 29 - 35 (2012/05/19)
Enzymatic modification can be used to enhance the bioactive properties of phenolic compounds. The present study employed laccase from Trametes pubescens to catalyze the modification of ferulic acid in a monophasic or biphasic system, as a way of enhancing
