35790-47-7Relevant articles and documents
Protein labeling approach to improve lysosomal targeting and efficacy of antibody-drug conjugates
Cai, Xiaoqing,Cheng, Yaping,Da, Yifan,Guo, Guoying,Han, Ying,Jiang, Xianxing,Li, Yan,Wang, Xin,Xiong, Jiale,Yu, Mingjia
, p. 3229 - 3233 (2020)
An anti-EGFR nanobody was labeled at the C-terminus with a lysosome-sorting NPGY (Asn-Pro-Gly-Tyr) motifviasortase-mediated ligation to enhance the engagement of the clathrin-mediated endocytosis. The synergistic effects of NPGY motif and nona-arginine peptide were found to induce robust internalization and lysosomal trafficking, which in turn improved anti-tumor activity of an antibody-drug conjugate.