3671-99-6

Usage

Chemical Properties

White powder

Uses

An inhibitor of HXK (hexokinase).

InChI:InChI=1/C6H13O9P.2Na.H2O/c7-3-2(1-14-16(11,12)13)15-6(10)5(9)4(3)8;;;/h2-10H,1H2,(H2,11,12,13);;;1H2/q;2*+1;/p-2/t2-,3-,4+,5-,6?;;;/m1.../s1

Upstream product

• 50-99-7 D-glucose 
• 26177-86-6 fructose 6-(disodium phosphate) 

Relevant academic research and scientific papers

Competitive inhibitors of yeast phosphoglucose isomerase: Synthesis and evaluation of new types of phosphorylated sugars from the synthon D-arabinonolactone-5-phosphate

Designed as competitive inhibitors of the isomerization reaction catalyzed by the potential chemotherapeutic target phosphoglucose isomerases (PGI), D-arabinonamide-5-phosphate and D-arabinonhydrazide-5-phosphate were synthesized and fully characterized. These new types of phosphorylated sugar derivatives were easily and efficiently obtained in a one-step procedure from the promising synthon D-arabinono-1,4-lactone 5-phosphate. These two compounds proved to be new good competitive inhibitors of yeast PGI with the substrate D-fructose-6-phosphate, though not as strong as D-arabinonhydroxamic acid-5-phosphate. Overall, our results are in accord with the postulated 1,2-cis-enediolate species as a probable high-energy intermediate of the PGI-catalyzed reaction. Copyright (C) 1999 Elsevier Science Ltd.

ENZYMATIC SYNTHESIS OF PHOSPHORIC MONOESTERS WITH ALKALINE PHOSPHATASE IN REVERSE HYDROLYSIS CONDITIONS

Title compounds were synthesized on a preparative scale using alkaline phosphatase, orthophosphoric monoester phosphohydrolase E.C. 3.1.3.1., in reverse hydrolysis conditions.Optimization for one of the 25 phosphoryl acceptors investigated (glycerol) shows that up to 55percent synthesis yield can be obtained using a large excess of substrate, conditions in which the enzymatic activity remains high.From the results obtained with different phosphoryl group donors, phosphate, pyrophosphate and polyphosphates and with enzymes of different sources, it comes up that the best results are obtained with pyrophosphate and with the weakly purified calf intestine alkaline phosphatase.The extent of enzymatic hydrolysis of the donor can be reduced owing to the existence of two different pH optima for the two reactions, phosphorylation and hydrolysis.The synthesis can be also performed using inert co-solvents which allow to reduce the amount of acceptor used, as long as Zn++ is added to the reaction medium.The results are discussed in terms of the catalytic mechanism of alkaline phosphatase.