38353-77-4Relevant academic research and scientific papers
RNA polymerase transcription promoters and nucleic acid sequencing method
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, (2008/06/13)
An RNA polymerase transcription accelerator comprising a compound represented by the following Formula (I) or salts thereof. A method of sequencing DNA in which nucleic acid transcripts are obtained using an RNA polymerase and a DNA fragment as a template, the resulted nucleic acid transcripts are separated, the nucleic acid sequence is determined from the separated fractions wherein the nucleic acid transcription reaction is carried out in the presence of a compound selected from a group of compounds represented by the above formula (I). The polyamine compounds above have outstanding accelerating activity on transcription activity of RNA polymerase. Therefore, use of the polyamine compounds in a DNA sequencing method using RNA polymerase can make a length of DNA sequence that can be determined in one sequencing longer.
Linear polyamine compounds and polyamine based anti-tumor agents
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, (2008/06/13)
Nobel polyamine compounds and polyamine compounds having carcinostatic action are provided. There are also provided anticancer agents containing as active ingredient at least one of polyamine compounds represented by the following Formula (I) or (III) and
Design and Synthesis of Macromonocyclic Polyamines Composed of Natural Methylene Arrays
Iwata, Masaaki,Kuzuhara, Hiroyoshi
, p. 198 - 210 (2007/10/02)
A general synthetic method applicable to acyclic and cyclic polyamines was developed.The methodology was exemplified by systematical synthesis of twelve macromonocyclic polyamines, 1 (N4) through 12 (N8), composed of the combination of four natural polyamine segments, spermidine, spermine, thermine, and thermospermine.These twelve designed macrocycles are exhausted numbers of possible structures defined by three arbitrarily chosen criteria concerning with methylene chain arrays and nitrogen content (four to eight).The common elements of the structural characteristics were analyzed and were found to be reduced to readily available three classes of simple N,N'-ditosylalkanediamines derived from diamines and triamine.Nitrogen content was increased systematically through the reaction of N,N'-ditosylalkanediamine with one of three ω-phthalimidated electrophiles followed by regeneration of the same functionality at symmetrical both terminals as the starting materials via a series of transformation reaction, in excellent yields.Tractable formamide intermediate profits the facile synthesis of acyclic polyamines with long chains.Cyclization was achieved, under high dilution conditions, through the reaction of α,ω-bis(tosylamide) with α,ω-ditosylates in DMF in the presence of cesium carbonate.The cyclization occurred in practical synthetic yields even in the formation of multi-membered ring when the shorter electrophile and the longest α,ω-bis(tosylamide) reacted.
Biosynthesis of blasticidin S from L-α-arginine. Stereochemistry in the arginine-2,3-aminomutase reaction
Prabhakaran,Woo,Yorgey,Gould
, p. 5785 - 5791 (2007/10/02)
A series of labeled α-arginines have been fed to fermentations of Streptomyces griseochromogenes in order to examine the mechanism of L-β-arginine formation in the biosynthesis of the antibiotic blasticidin S. [3-13C,2-15N]Arginine was synthesized and fed; analysis of the derived antibiotic by 13C NMR spectroscopy revealed the retention of the original α-nitrogen and its intramolecular migration to the β-position, revealing the presence of an arginine-2,3-aminomutase. Feedings of [2,3,3-2H3]-, [3,3-2H2]-, and [2-2H]arginines revealed the complete retention of the original β-hydrogens with migration of one to the α-position, as well as partial loss of the original α-hydrogen presumably due to arginine racemase activity. (3R)-[3-2H]- and (3S)-[3-2H]arginines were synthesized unambiguously and used to determine that the pro-3R hydrogen of α-arginine migrates to the α-position (C-2). δ-N-[13CH3]Methylarginine was synthesized, mixed with [guanidino-14C]arginine, and fed to S. griseochromogenes. A 42% incorporation of radioactivity from arginine was obtained, but no 13C enrichment was observed in the blasticidin S sample, indicating that arginine, itself, is the aminomutase substrate.
The biosynthesis of the streptolidine moiety in streptothricin F
Martinkus,Tann,Gould
, p. 3493 - 3505 (2007/10/02)
A series of arginines specifically labeled either with 13C and 15N or with 2H were synthesized and fed to Streptomyces L-1689-23. The streptothricin F isolated in each case was analyzed by either 13C or 2H NMR, respectively, in order to determine the labeling pattern obtained. From these results, it appears that arginine is metabolized to a β-ketoarginine, possibly via a pyridoxal phosphate adduct, and then via cyclization, reduction, rearrangement, and hydroxylation to the streptolidine moiety. The pathway described can also account for the formation of other known antibiotics, and for β-hydroxy-γ-amino acids, generally.
