389625-50-7

Basic information

• Product Name: 2'-chloro-5'-bromomethylfluorescein di-t-butyldimethylsilyl ether
• Synonyms: 2'-chloro-5'-bromomethylfluorescein di-t-butyldimethylsilyl ether
• CAS NO: 389625-50-7
• Molecular Weight: 688.206
• Mol File: 389625-50-7.mol

Chemical Properties

• CAS DataBase Reference: 2'-chloro-5'-bromomethylfluorescein di-t-butyldimethylsilyl ether(CAS DataBase Reference)
• NIST Chemistry Reference: 2'-chloro-5'-bromomethylfluorescein di-t-butyldimethylsilyl ether(389625-50-7)
• EPA Substance Registry System: 2'-chloro-5'-bromomethylfluorescein di-t-butyldimethylsilyl ether(389625-50-7)

Safety Data

• Hazardous Substances Data: 389625-50-7(Hazardous Substances Data)

Upstream product

• 85-44-9 phthalic anhydride 

Downstream Products

• 502467-19-8 C₅₂H₅₉ClN₄O₅Si₂ 

Relevant articles and documents

ZP4, an improved neuronal Zn2+ sensor of the Zinpyr family

A second-generation fluorescent sensor for Zn2+ from the Zinpyr family, ZP4, has been synthesized and characterized, ZP4 (Zinpyr-4, 9-(o-carboxyphenyl)-2-chloro-5-[2-{bis(2-pyridylmethyl)-aminomethyl}- N-methylaniline]-6-hydroxy-3-xanthanone) is prepared via a convergent synthetic strategy developed from previous studies with these compounds, ZP4, like its predecessors, has excitation and emission wavelengths in the visible range (～500 nm), a dissociation constant (Kd) for Zn2+ of less than 1 nM and a high quantum yields (Π = ～0.4), making it well suited for biological applications, A 5-fold fluorescent enhancement is observed under simulated physiological conditions corresponding to the binding of the Zn2+ cation to the sensor, which inhibits a photoinduced electron transfer (PET) quenching pathway. The metal-binding stereochemistry of ZP4 was evaluated through the synthesis and X-ray structural characterization of [M(BPAMP)(H2O)n]+ complexes, where BPAMP is [2-{bis(2-pyridylmethyl)aminomethyl}-N-methylaniline]-phenol and M = Mn2+, Zn2+ (n = 1) or Cu2+ (n = 0).