4004-05-1 Usage
Uses
Used in Pharmaceutical Applications:
1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) is used as a component in the formation of heterogeneous liposomes with N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium methylsulfate (DOTAP). These liposomes serve as delivery vehicles for therapeutic agents, enhancing the efficiency of drug administration.
Used in Gene Delivery:
DOPE is used as an emulsifier to facilitate the transport of DNA-liposome complexes across membranes. It is often used in combination with cationic phospholipids to increase the efficiency of DNA transfection studies, providing a non-viral method of gene delivery.
Used in Biomedical Research:
DOPE has been utilized in the preparation of liposomes for various research applications, such as the reconstitution of human uncoupling protein 1 (UCP1) and the conjugation of Caenorhabditis elegans autophagy-related cysteine protease proteins. Additionally, it has been used in the generation of giant unilamellar vesicles (GUVs) for studying septin-induced deformation.
Used in the Preparation of Liposomes and Giant Unilamellar Vesicles (GUVs):
DOPE is employed in the preparation of liposomes and GUVs due to its ability to form different structures based on pH changes, making it a versatile component in the study of membrane biophysics and the development of novel drug delivery systems.
Biological Activity
DOPE in mixed membranes have been found to decrease fluorescence lifetime and increase acyl-chain order. DOPE is an essential component of cationic liposomes designed to deliver DNA into gliosarcoma and kidney cell lines (gene therapy).
Biochem/physiol Actions
DOPE in mixed membranes have been found to decrease fluorescence lifetime and increase acyl-chain order. DOPE is an essential component of cationic liposomes designed to deliver DNA into gliosarcoma and kidney cell lines (gene therapy).
Check Digit Verification of cas no
The CAS Registry Mumber 4004-05-1 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 4,0,0 and 4 respectively; the second part has 2 digits, 0 and 5 respectively.
Calculate Digit Verification of CAS Registry Number 4004-05:
(6*4)+(5*0)+(4*0)+(3*4)+(2*0)+(1*5)=41
41 % 10 = 1
So 4004-05-1 is a valid CAS Registry Number.
InChI:InChI=1/C41H78NO8P/c1-3-5-7-9-11-13-15-17-19-21-23-25-27-29-31-33-40(43)47-37-39(38-49-51(45,46)48-36-35-42)50-41(44)34-32-30-28-26-24-22-20-18-16-14-12-10-8-6-4-2/h17-20,39H,3-16,21-38,42H2,1-2H3,(H,45,46)/b19-17-,20-18-/t39-/m1/s1
4004-05-1Relevant articles and documents
TREATMENT OF CYSTIC FIBROSIS BY DELIVERY OF NEBULIZED mRNA ENCODING CFTR
-
, (2020/06/08)
The present invention provides, among other things, an improved method of treating cystic fibrosis (CF) in a human subject. The method comprises administering a composition comprising an mRNA encoding a Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) protein at a concentration of 0.5 mg/mL or greater to a human subject via nebulization. The composition is aerosolized using a nebulizer and a nominal dose of the mRNA is administered to the human subject via the nebulizer over a period of time, typically at least 30 minutes, at a suitable nebulization rate, e.g., at least 0.2 mL/minute.
The development of improved cationic lipids for gene transfer into cystic fibrosis airway epithelial cells
Harris,Marshall,Lee,Siegel,Mcneilly,Yew,Nietupski,Nichols,Cherry,Wan,Jiang,Lane,Rowe,Scheule,Smith,Cheng
, p. 289 - 292 (2007/10/03)
In an effort to improve the efficiency of cationic lipid mediated gene transfer, over 90 novel cationic lipids of diverse structural types were synthesized and evaluated in vitro. Four cationic lipids derived from phospholipids were examined. The most promising cationic lipid formulations were tested in vivo by intranasal or transtracheal instillation into the lungs of BALB/c mice. The most active formulations gave CAT reporter gene expression levels which are greater than 500 fold over that which could be attained using free DNA alone. Certain cationic lipid formulations have been shown to facilitate substantial expression of the CFTR (cystic fibrosis transmembrane conductance regulator) gene in vitro as determined by the SPQ and Ussing Chamber assays.
Synthesis of New Phospholipids Linked to Steroid-Hormone Derivatives Designed for Two-Dimensional Crystallization of Proteins
Lebeau, Luc,Oudet, Pierre,Mioskowski, Charles
, p. 1697 - 1706 (2007/10/02)
The synthesis of phospholipids 1n-3n, rationally designed for two-dimensional crystallization of progesterone and estradiol receptors, is reported.The structure of theses lipids provides them with essential properties such as fluidity and stability when spread into monolayers at the air/H2O interface, affinity for the protein to be crystallized, and accessibility of the ligand under the lipid monolayer.