403501-31-5Relevant academic research and scientific papers
Enhanced Turnover for the P450 119 Peroxygenase-Catalyzed Asymmetric Epoxidation of Styrenes by Random Mutagenesis
Wang, Li,Wei, Siping,Pan, Xianchao,Liu, Pingxian,Du, Xi,Zhang, Chun,Pu, Lin,Wang, Qin
, p. 2741 - 2749 (2018/02/28)
A randomized library is constructed based on pET30a-CYP119-T214V plasmid. This library of random mutants of CYP119-T214V was screened by means of the reduced CO difference spectra and epoxidation of styrene. By using directed evolution, a new CYP119 quadr
Engineering P450 Peroxygenase to Catalyze Highly Enantioselective Epoxidation of cis-β-Methylstyrenes
Zhang, Chun,Liu, Ping-Xian,Huang, Lu-Yi,Wei, Si-Ping,Wang, Li,Yang, Sheng-Yong,Yu, Xiao-Qi,Pu, Lin,Wang, Qin
, p. 10969 - 10975 (2016/07/27)
P450 119 peroxygenase and its site-directed mutants are discovered to catalyze the enantioselective epoxidation of methyl-substituted styrenes. Two new site-directed P450 119 mutants, namely T213Y and T213M, which were designed to improve the enantioselectivity and activity for the epoxidation of styrene and its methyl substituted derivatives, were studied. The T213M mutant is found to be the first engineered P450 peroxygenase that shows highly enantioselective epoxidation of cis-β-methylstyrenes, with up to 91 % ee. Molecular modeling studies provide insights into the different catalytic activity of the T213M mutant and the T213Y mutant in the epoxidation of cis-β-methylstyrene. The results of the calculations also contribute to a better understanding of the substrate specificity and configuration control for the regio- and stereoselective peroxygenation catalyzed by the T213M mutant.
Asymmetric epoxidation of cis-β-methylstyrenes catalyzed by N-aryl substituted oxazolidinone-containing ketones. A beneficial substituent effect
Shu, Lianhe,Shi, Yian
, p. 8115 - 8117 (2007/10/03)
Asymmetric epoxidation of substituted cis-β-methylstyrenes using N-aryl substituted oxazolidinone-containing ketones as catalysts shows that substituents on the phenyl group of the olefin have significant positive effects on the enantioselectivity of the
Designing new chiral ketone catalysts. Asymmetric epoxidation of cis-olefins and terminal olefins
Tian, Hongqi,She, Xuegong,Yu, Hongwu,Shu, Lianhe,Shi, Yian
, p. 2435 - 2446 (2007/10/03)
This paper describes a new class of chiral oxazolidinone ketone catalyst for asymmetric epoxidation. High ee values have been obtained for a number of cyclic and acyclic cis-olefins. The epoxidation was stereospecific with no isomerization observed in the epoxidation of acyclic systems. Encouragingly high ee values have also been obtained for a number of terminal olefins. Mechanistic studies show that electronic interactions play an important role in stereodifferentiation.
