407618-07-9Relevant academic research and scientific papers
A Universal Labeling Strategy for Nucleic Acids in Expansion Microscopy
Wen, Gang,Vanheusden, Marisa,Leen, Volker,Rohand, Taoufik,Vandereyken, Katy,Voet, Thierry,Hofkens, Johan
supporting information, p. 13782 - 13789 (2021/09/11)
Expansion microscopy (ExM) enables the nanoscale imaging of ribonucleic acids (RNAs) on a conventional fluorescence microscope, providing information on the intricate patterns of gene expression at (sub)cellular resolution and within spatial context. To extend the use of such strategies, we examined a series of multivalent reagents that allow the labeling and grafting of deoxyribonucleic acid (DNA) oligonucleotide probes in a unified approach. We show that the reagents are directly compatible with third-generation in situ hybridization chain reaction RNA FISH (fluorescence in situ hybridization) techniques while displaying complete retention of the targeted transcripts. Furthermore, we validate and demonstrate that our labeling method is compatible with multicolor staining. Through oligonucleotide-conjugated antibodies, we demonstrate excellent performance in ×4 ExM and ×10 ExM, achieving a resolution of ～50 nm in ×10 ExM for both pre- and postexpansion labeling strategies. Our results indicate that our multivalent molecules enable the rapid functionalization of DNA oligonucleotides for ExM.
Divergent synthesis of photoaffinity probe candidates by click reactions of azido-substituted aryltrifluoromethyldiazirines
Watanabe, Kenji,Tsuda, Junpei,Ochiai, Hidenori,Niwa, Takashi,Hosoya, Takamitsu
, p. 1366 - 1387 (2019/07/31)
Two types of azido-substituted aryltrifluoromethyldiazirine units have been designed and prepared for the divergent synthesis of photoaffinity probe (PAP) candidates. Using these azides, various aryltrifluoromethyldiazirine derivatives have been rendered easily synthesizable by several click reactions, as well as the Staudinger reduction affording the corresponding aniline. The triazole-conjugated aryltrifluoromethyldiazirine derivatives prepared in this study showed normal photoreactivity compared with those reported previously. These results indicate the utility of these azido-substituted aryltrifluoromethyldiazirine units for development of PAPs for target identification of bioactive compounds.
NOVEL N-BENZYLAMIDE SUBSTITUTED DERIVATIVES OF 2-(ACYLAMIDO)ACETIC ACID AND 2-(ACYLAMIDO)PROPIONIC ACIDS: POTENT NEUROLOGICAL AGENTS
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, (2009/12/27)
A first aspect of the invention is a compound (sometimes also referred to herein as an "active agent" or "active compound") of Formula (I) or ( Ia): or a pharmaceutically acceptable salt or prodrug thereof. Compositions thereof and methods of using the same (e.g. for the treatment of a neurological disease) are also described.
