87736-86-5Relevant articles and documents
Ammonia-free synthesis of 3-trifluoromethyl-3-phenyldiaziridine
Kumar, Arun Babu,Manetsch, Roman
, p. 626 - 631 (2018/02/26)
An ammonia-free synthesis of 3-trifluoromethyl-3-phenyldiaziridine, an important intermediate in the synthesis of a widely used photolabel 3-trifluoromethyl-3-phenyldiazirine, is described. By avoiding the use of volatile, corrosive, and toxic anhydrous ammonia, the major hazard involved in the synthesis of this widely used photolabel is eliminated. Furthermore, this synthesis is convenient compared to the conventional route, since it is significantly less time consuming and, due to the absence of liquid ammonia, this method does not require the maintenance of low temperature for prolonged periods.
Diazirine-containing RNA photo-cross-linking probes for capturing microRNA targets
Nakamoto, Kosuke,Ueno, Yoshihito
, p. 2463 - 2472 (2014/04/17)
Here, we report the applicability of diazirine-containing RNA photo-cross-linking probes for the identification of microRNA (miRNA) targets. The RNA cross-linking probes were synthesized by substituting the RNA nucleobases with nucleoside analogues such as 1-O-[3-(3-trifluoromethyl-3H- diazirin-3-yl)]benzyl-β-d-ribofuranose or 1-O-[4-(3-trifluoromethyl-3H- diazirin-3-yl)]benzyl-β-d-ribofuranose that carry aryl trifluoromethyl diazirine moieties. The probes were successfully cross-linked with synthetic RNAs containing the four natural nucleosides on the opposite site of the nucleoside analogues. Furthermore, it was found that miRNAs containing these analogues were effective in regulating the expression of their target genes. Thus, RNAs containing the nucleoside analogues are promising candidates as photo-cross-linking probes to identify the target mRNAs of miRNAs.
Trifluoromethyldiazirine: An effective photo-induced cross-linking probe for exploring amyloid formation
Smith, David P.,Anderson, Jon,Plante, Jeffrey,Ashcroft, Alison E.,Radford, Sheena E.,Wilson, Andrew J.,Parker, Martin J.
supporting information; experimental part, p. 5728 - 5730 (2009/04/13)
The separative and analytical power of ion mobility spectrometry-mass spectrometry combined with photo-induced cross-linking of site-specifically incorporated trifluoromethyldiazirine provides a powerful approach towards structural characterisation of amy
