41613-02-9Relevant articles and documents
Quantitative analysis of phytate globoids isolated from wheat bran and characterization of their sequential dephosphorylation by wheat phytase
Bohn, Lisbeth,Josefsen, Lone,Meyer, Anne S.,Rasmussen, Soren K.
, p. 7547 - 7552 (2008/09/19)
Wheat phytase was purified to investigate the action of the enzyme toward its pure substrate (phytic acid - myo-inositol hexakisphosphate) and its naturally occurring substrate (phytate globoids). Phytate globoids were purified to homogeneity from wheat bran, and their nutritionally relevant parameters were quantified by ICP-MS. The main components of the globoids were phytic acid (40% w/w), protein (46% w/w), and several minerals, in particular, K > Mg > Ca > Fe (in concentration order). Investigation of enzyme kinetics revealed that Km and Vmax decreased by 29 and 37%, respectively, when pure phytic acid was replaced with phytate globoids as substrate. Time course degradation of phytic acid or phytate globoids using purified wheat phytase was followed by HPIC identification of inositol phosphates appearing and disappearing as products. In both cases, enzymatic degradation initiated at both the 3- and 6-positions of phytic acid and end products were inositol and phosphate.
De novo synthesis of the enantiomers Ins(1,2,3,4)P4 and Ins(1,2,3,6)P4-regiospecificity of their enzymatic dephosphorylation
Plettenburg,Adelt,Vogel,Altenbach
, p. 1057 - 1061 (2007/10/03)
The first total synthesis of Ins(1,2,3,4)P4 and Ins(1,2,3,6)P4 is presented. Starting from p-benzoquinone, we took advantage of the C2-symmetry of conduritol-B intermediates. The target compounds were dephosphorylated by s
The First Synthesis and Iron Binding Studies of the Natural Product, myo-Inositol 1,2,3-Trisphosphate
Spiers, Ian D.,Freeman, Sally,Poyner, David R.,Schwalbe, Carl H.
, p. 2125 - 2128 (2007/10/02)
The natural product myo-inositol 1,2,3-trisphosphate 1 has been prepared and shown to inhibit Fe3+-catalysed hydroxyl radical formation.
