42871-95-4Relevant academic research and scientific papers
Bioconjugatable azo-based dark-quencher dyes: Synthesis and application to protease-activatable far-red fluorescent probes
Chevalier, Arnaud,Massif, Cédrik,Renard, Pierre-Yves,Romieu, Anthony
, p. 1686 - 1699 (2013/04/10)
We describe the efficient synthesis and one-step derivatization of novel, nonfluorescent azo dyes based on the Black Hole Quencher-3 (BHQ-3) scaffold. These dyes were equipped with various reactive and/or bioconjugatable groups (azido, α-iodoacetyl, keton
Diol appended quenchers for fluorescein boronic acid
Elfeky, Souad A.,Flower, Stephen E.,Masumoto, Naoko,D'hooge, Francois,Labarthe, Ludivine,Chen, Wenbo,Len, Christophe,James, Tony D.,Fossey, John S.
experimental part, p. 581 - 588 (2010/08/19)
Fluorescein isothiocyanate is treated with 3-aminophenylboronic acid to provide a fluorescently tagged boronic acid derivative which is used to assess Forster resonance energy transfer (FRET) quenching upon boronate ester formation with a series of bespoke diol appended quenchers. Fluorescence spectroscopy comparison of quenching efficiency between treatment of fluorescein and its boronic acid appended congener with quencher appended diol reveals boronate ester formation (covalently linked) to be the more efficient regime and from the panel of quenchers which also included nucleosides.
