438194-50-4Relevant academic research and scientific papers
Immobilisation on polystyrene of diazirine derivatives of mono- and disaccharides: Biological activities of modified surfaces
Chevolot,Martins,Milosevic,Leonard,Zeng,Malissard,Berger,Maier,Mathieu,Crout,Sigrist
, p. 2943 - 2953 (2001)
The potential of surface glycoengineering for biomaterials and biosensors originates from the importance of carbohydrate protein interactions in biological systems. The strategy employed here utilises carbene generated by illumination of diazirine to achieve covalent bonding of carbohydrates. Here, we describe the synthesis of an aryl diazirine containing a disaccharide (lac-tose). Surface analysis techniques [X-ray photoelectron spectroscopy (XPS) and time of flight secondary ion mass spectroscopy (ToF-SIMS)] demonstrate its successful surface immobilisation on polystyrene (PS). Results are compared to those previously obtained with an aryl diazirine containing a monosaccharide (galactose). The biological activity of galactose- or lactose-modified PS samples is studied using rat hepatocytes, Allo A lectin and solid-phase semi-synthesis with α-2,6-sialyltransferase. Allo A shows some binding to galactose-modified PS but none to lactose-modified surfaces. Similar results are obtained with rat hepatocytes. In contrast, sialylation of lactose-modified PS is achieved but not with galactose-modified surfaces. The different responses indicate that the biological activity depends not only on the carbohydrate per se but also on the structure and length of the spacer. Copyright
Process for the modification of surface
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Page column 8-9, (2010/02/08)
The present invention relates to a process for the modification of a material surface comprising the steps of (a1) photochemically fixing one or more different compounds of formula onto the material surface, or (a2) photochemically f
