4416-57-3

Basic information

• Product Name: testololactone
• Synonyms: testololactone;17a-Oxa-D-homoandrosta-4-ene-3,17-dione;D-Homo-17a-oxaandrost-4-ene-3,17-dione;(4aS,4bR,10aR,10bS,12aS)-10a,12a-dimethyl-4,4a,4b,5,6,9,10,10b,11,12-decahydro-3H-naphtho[2,1-f]chromene-2,8-dione
• CAS NO: 4416-57-3
• Molecular Formula: C₁₉H₂₆O₃
• Molecular Weight: 302.411
• Mol File: 4416-57-3.mol
• Article Data: 6

Chemical Properties

• Melting Point: 207-209 °C
• Boiling Point: 478.2°Cat760mmHg
• Flash Point: 211.2°C
• Appearance: /
• Density: 1.15g/cm³
• Vapor Pressure: 2.62E-09mmHg at 25°C
• Refractive Index: 1.549
• Storage Temp.: Refrigerator
• Solubility: Chloroform (Slightly), DMSO (Slightly)
• CAS DataBase Reference: testololactone(CAS DataBase Reference)
• NIST Chemistry Reference: testololactone(4416-57-3)
• EPA Substance Registry System: testololactone(4416-57-3)

Safety Data

• Hazardous Substances Data: 4416-57-3(Hazardous Substances Data)

Usage

Uses

Testololactone, is the derivative of Testolactone (T154800), which is an antineoplastic agent that is a derivative of progesterone and is used to treat advanced stage breast cancer.

InChI:InChI=1/C19H26O3/c1-18-9-7-13(20)11-12(18)3-4-14-15(18)8-10-19(2)16(14)5-6-17(21)22-19/h11,14-16H,3-10H2,1-2H3/t14-,15+,16+,18+,19+/m1/s1

Upstream product

• 57-83-0 Progesterone 
• 145-13-1 Pregnenolone 
• 53-43-0 dehydroepiandrosterone 
• 521-17-5 5-androstenediol 

Relevant articles and documents

Biotransformations of steroids to testololactone by a multifunctional strain Penicillium simplicissimum WY134-2

The biotransformations of a range of steroidal compounds, including 17α-hydroxy progesterone, progesterone, testosterone, androst-4-ene-3,17- dione (AD), pregnenolone, and dehydroepiandrosterone (DHEA), by Penicillium simplicissimum WY134-2 have been investigated. In all the cases, testolic acid and testololactone were detected, and the acid was converted to the lactone when pH was adjusted to 1, leading to isolation of testololactone in 25%-96% yields. Especially for progesterone and testosterone, the isolated yields were 93% and 96% with substrate concentration being 3 g/L, suggesting that P. simplicissimum WY134-2 may be used for the synthesis of testololactone. The results revealed the multi-functional catalytic activity of P. simplicissimum WY134-2 toward steroids for the first time. The possible reaction pathways of steroids promoted by this strain were discussed.

Studies on Baeyer-Villiger oxidation of steroids: DHEA and pregnenolone d-lactonization pathways in Penicillium camemberti AM83

Penicillium camemberti AM83 strain is able to carry out effective Baeyer-Villiger type oxidation of DHEA, pregnenolone, androstenedione and progesterone to testololactone. Pregnenolone and DHEA underwent oxidation to testololactone via two routes: through

Baeyer-Villiger oxidation of DHEA, pregnenolone, and androstenedione by Penicillium lilacinum AM111

The Baeyer-Villiger monooxygenase (BVMO) produced by Penicillium lilacinum AM111, in contrast to other enzymes of this group known in the literature, is able to process 3β-hydroxy-5-ene steroid substrates. Transformation of DHEA and pregnenolone yielded, as a sole or main product, 3β-hydroxy-17a-oxa-d-homo-androst-5-en-17-one, a new metabolite of these substrates; pregnenolone was transformed also to testololactone. Testololactone was the only product of oxidation of androstenedione by P. lilacinum AM111. Investigations of the time evolution of reaction progress have indicated that the substrates stimulate activity of BVMO(s) of P. lilacinum AM111.