4439-05-8

Basic information

• Product Name: BASIC FUCHSIN LEUCONITRILE
• Synonyms: BASIC FUCHSIN LEUCONITRILE;TRIS-(4-AMINOPHENYL)-ACETONITRILE;Pararosaniline leuconitrile
• CAS NO: 4439-05-8
• Molecular Formula: C₂₀H₁₈N₄
• Molecular Weight: 314.38
• Mol File: 4439-05-8.mol

Chemical Properties

• Boiling Point: 566.8 °C at 760 mmHg
• Flash Point: 296.6 °C
• Appearance: /
• Density: 1.259 g/cm³
• Vapor Pressure: 7.26E-13mmHg at 25°C
• Refractive Index: 1.703
• CAS DataBase Reference: BASIC FUCHSIN LEUCONITRILE(CAS DataBase Reference)
• NIST Chemistry Reference: BASIC FUCHSIN LEUCONITRILE(4439-05-8)
• EPA Substance Registry System: BASIC FUCHSIN LEUCONITRILE(4439-05-8)

Safety Data

• Hazardous Substances Data: 4439-05-8(Hazardous Substances Data)

Usage

Uses

Used in Histology:
Basic fuchsin leuconitrile is used as a staining agent for visualizing cellular structures in histological samples. Its cationic nature allows it to bind with negatively charged components, enhancing the contrast and clarity of microscopic images.
Used in Microbiology:
In the field of microbiology, Basic fuchsin leuconitrile is used as a differential stain for various purposes:
1. Gram Stain Technique:
Basic fuchsin leuconitrile is used as a counterstain in the Gram stain technique to differentiate between Gram-positive and Gram-negative bacteria based on their cell wall compositions. After the initial staining with crystal violet, the dye binds to the decolorized Gram-negative bacteria, providing a red color that helps in their identification.
2. Ziehl-Neelsen Stain:
Basic fuchsin leuconitrile is used in the Ziehl-Neelsen stain for the detection of acid-fast bacteria, such as Mycobacterium tuberculosis. After the initial staining with carbolfuchsin, the dye is used as a counterstain to color the background blue, allowing the acid-fast bacteria to stand out as red, facilitating their identification.

InChI:InChI=1/C20H18N4/c21-13-20(14-1-7-17(22)8-2-14,15-3-9-18(23)10-4-15)16-5-11-19(24)12-6-16/h1-12H,22-24H2

Upstream product

• 151-50-8 potassium cyanide 
• 4955-92-4 pararosaniline hydrochloride 
• 569-61-9 basic red 9 
• 596-48-5 tris(p-nitrophenyl)carbinol 
• 6639-43-6 2,2,2-triphenylacetonitrile 
• 7732-18-5 water 
• 861296-52-8 tris-(4-nitro-phenyl)-acetonitrile 

Downstream Products

• 307351-60-6 tris-[4-(trityl-amino)-phenyl]-acetonitrile 
• 467-62-9 pararosaniline 
• 6639-43-6 2,2,2-triphenylacetonitrile 

Relevant articles and documents

Photochemistry and photophysics of triarylmethane dye leuconitriles

The photochemical reactions of crystal violet leuconitrile (CVCN) were investigated by the means of product analysis and trapping experiments, laser flash and steady-state photolysis, and steady-state fluorescence. The influence of oxygen on the reaction was examined in detail. The photochemistry of malachite green leuconitrile (MGCN), basic fuchsin leuconitrile (BFCN), and crystal violet leucomethyl (CVMe) and leucobenzyl (CVBn), as well as triphenylacetonitrile, was studied. The results suggest ionization occurs from S1, while the di-π-methane reaction is the photochemical route from T1.