4483-62-9Relevant academic research and scientific papers
A chromogenic substrate for solid-phase detection of phospholipase A 2
Eba, Chisato,Okano, Aoi,Nakano, Hideo,Iwasaki, Yugo
, p. 43 - 45 (2014/01/17)
A method for solid-phase detection of phospholipase A2 (PLA 2) was developed. The method uses 1-octanoyloxynaphthalene-3-sulfonic acid, which was found to be a good substrate of PLA2. The substrate is hydrolyzed by PLA2 into 1-naphthol-3-sulfonic acid, which is spontaneously coupled with coexisting diazonium salt to form a red-purple azo dye. Streptomyces and bovine pancreatic PLA2 spotted on a nitrocellulose membrane could be detected by this method with considerable sensitivity. In addition, colonies of recombinant Escherichia coli producing bacterial PLA2 were distinguishable from those producing an inactive mutant PLA2, facilitating high-throughput screening in directed evolution of the enzyme.
Manufacture of higher aryl esters
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, (2008/06/13)
The oxidation process for the manufacture of higher aryl esters comprising contacting the reaction mixture of a higher aromatic hydrocarbon, an organic solvent, a carboxylic acid and molecular oxygen in the liquid phase at an elevated temperature with a catalyst composed of palladium or a compound of palladium, an antimony compound and a compound of at least one member selected from the group consisting of alkali metals and alkaline earth metals is described.
