475578-64-4Relevant academic research and scientific papers
A Copper Nanocluster-Based Fluorescent Probe for Real-Time Imaging and Ratiometric Biosensing of Calcium Ions in Neurons
Liu, Zhichao,Jing, Xia,Zhang, Sanjun,Tian, Yang
, p. 2488 - 2497 (2019)
Fluorescent calcium ion (Ca2+) sensing and imaging have become an essential technique for investigation of signaling pathways of Ca2+ and understanding the role of Ca2+ in neurodegenerative disease. Herein a copper nanocluster (CuNC)-based ratiometric fluorescent probe was developed for real-time sensing and imaging of Ca2+ in neurons, in which a specific Ca2+ ligand with two formaldehyde groups was synthesized and further conjugated with polyethylenimine (PEI) to form a new ligand molecule for the synthesis of CuNCs. Meanwhile, water-soluble Alex Fluor 660 NHS ester was immobilized onto CuNCs as a reference element. The developed ratiometric fluorescence nanoprobe demonstrated a good linearity with Ca2+ concentration in the range of 2-350 μM, and a detection limit down to 220 ± 11 nM was achieved. In addition, the response time of the present probe for Ca2+ was found to be less than 2 s with good stability and high selectivity. Taking advantage of the low cytotoxicity and good biocompatibility of the developed nanoprobe, it was discovered that the histamine-induced cytoplasmic Ca2+ increase in various parts of neurons was different. Moreover, it was found O2--induced cytoplasmic Ca2+ burst and O2--induced neuronal death possibly resulted from Ca2+ overload in the neurons.
Long-Term Tracking and Dynamically Quantifying of Reversible Changes of Extracellular Ca2+ in Multiple Brain Regions of Freely Moving Animals
Liu, Yuandong,Liu, Zhichao,Zhao, Fan,Tian, Yang
, p. 14429 - 14437 (2021)
Understanding physiological and pathological processes in the brain requires tracking the reversible changes in chemical signals with long-term stability. We developed a new anti-biofouling microfiber array to real-time quantify extracellular Ca2+ concentrations together with neuron activity across many regions in the mammalian brain for 60 days, in which the signal degradation was 2+ upon ischemia-reperfusion processes. The changing sequence and rate of Ca2+ in 7 brain regions were different during the stroke. ROS scavenger could protect Ca2+ influx and neuronal activity after stroke, suggesting the significant influence of ROS on Ca2+ overload and neuron death. We demonstrated this microarray is a versatile tool for investigating brain dynamic during pathological processes and drug treatment.
A membrane permeable fluorescent Ca2+ probe based on bis-BODIPY with branched PEG
Duan, Wenfeng,Wei, Huimin,Cui, Tengbo,Gao, Baoxiang
supporting information, p. 894 - 898 (2015/02/05)
A new class of Ca2+ probes is developed based on PEG-BODIPY-BAPTA conjugates. These newly formed probes with BAPTA acid exhibit high sensitivity and selectivity for Ca2+ over other metal ions, can pass through the cell membranes of living cells without special procedures, and can monitor changes in the intracellular Ca2+ signal.
Ultralow calcium requirements of fungi facilitate use of calcium regulating agents to suppress host calcium-dependent defenses, synergizing infection by a mycoherbicide
Gressel, Jonathan,Michaeli, Daphna,Kampel, Vladimir,Amsellem, Ziva,Warshawsky, Abraham
, p. 6353 - 6360 (2007/10/03)
Infection by many fungi activates a variety of calcium dependent defenses in the hosts, slowing or suppressing the attacker and limiting the efficacy of mycoherbicides. The calcium requirement for fungal growth is so low that it could only be implied based on fungi containing calcium-dependent signaling enzymes. Analytical grade media contain 2 μM calcium, and the addition of specific chelators does not affect fungal growth. Hydrophobic derivatives of the calcium-specific chelator BAPTA designed to traverse plant cuticles were synthesized in order to chelate calcium internally during fungal attack. Some chelators as well as calcium precipitating oxalate and channel blocker verapamil were applied with a weakly mycoherbicidal Colletotrichum coccodes to cotyledons of compatible Abutilon threophrasti. They suppressed calcium dependent callose biosynthesis in the weed and increased virulence but may have affected other calcium-dependent processes that facilitate virulence. The low calcium requirement of fungi, and their high affinity for calcium, allows the application of calcium-regulating agents as synergists for mycoherbicides where the weed uses calcium-dependent defenses.
