475578-64-4

Basic information

• Product Name: 5,5’-diformyl-1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic ethyl ester
• Synonyms: 5,5’-diformyl-1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic ethyl ester
• CAS NO: 475578-64-4
• Molecular Weight: 644.676
• Mol File: 475578-64-4.mol
• Article Data: 4

Chemical Properties

• CAS DataBase Reference: 5,5’-diformyl-1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic ethyl ester(CAS DataBase Reference)
• NIST Chemistry Reference: 5,5’-diformyl-1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic ethyl ester(475578-64-4)
• EPA Substance Registry System: 5,5’-diformyl-1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic ethyl ester(475578-64-4)

Safety Data

• Hazardous Substances Data: 475578-64-4(Hazardous Substances Data)

Upstream product

• 51661-19-9 1,2-bis(2-nitrophenoxy)ethane 
• 88-75-5 2-hydroxynitrobenzene 
• 52411-34-4 1,2-Bis(2-aminophenoxy)ethane 
• 68-12-2 N,N-dimethyl-formamide 
• 73630-07-6 1,2-bis(2-aminophenoxy)ethane-N,N,N'N'-tetraacetic acid tetraethyl ester 

Downstream Products

• 475578-63-3 5,5'-di(benzyliminomethyl)-BAPTA-tetraethyl ester 
• 1417178-69-8 C₆₆H₆₈N₆O₁₄ 

Relevant articles and documents

A Copper Nanocluster-Based Fluorescent Probe for Real-Time Imaging and Ratiometric Biosensing of Calcium Ions in Neurons

Fluorescent calcium ion (Ca2+) sensing and imaging have become an essential technique for investigation of signaling pathways of Ca2+ and understanding the role of Ca2+ in neurodegenerative disease. Herein a copper nanocluster (CuNC)-based ratiometric fluorescent probe was developed for real-time sensing and imaging of Ca2+ in neurons, in which a specific Ca2+ ligand with two formaldehyde groups was synthesized and further conjugated with polyethylenimine (PEI) to form a new ligand molecule for the synthesis of CuNCs. Meanwhile, water-soluble Alex Fluor 660 NHS ester was immobilized onto CuNCs as a reference element. The developed ratiometric fluorescence nanoprobe demonstrated a good linearity with Ca2+ concentration in the range of 2-350 μM, and a detection limit down to 220 ± 11 nM was achieved. In addition, the response time of the present probe for Ca2+ was found to be less than 2 s with good stability and high selectivity. Taking advantage of the low cytotoxicity and good biocompatibility of the developed nanoprobe, it was discovered that the histamine-induced cytoplasmic Ca2+ increase in various parts of neurons was different. Moreover, it was found O2--induced cytoplasmic Ca2+ burst and O2--induced neuronal death possibly resulted from Ca2+ overload in the neurons.

A membrane permeable fluorescent Ca2+ probe based on bis-BODIPY with branched PEG

A new class of Ca2+ probes is developed based on PEG-BODIPY-BAPTA conjugates. These newly formed probes with BAPTA acid exhibit high sensitivity and selectivity for Ca2+ over other metal ions, can pass through the cell membranes of living cells without special procedures, and can monitor changes in the intracellular Ca2+ signal.