47575-61-1Relevant articles and documents
Estriol hapten and antibody and application thereof in fast sensor method for uncojugated estriol detection
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Paragraph 0052; 0053; 0054, (2016/10/27)
The invention relates to the technical field of biosensors and discloses an estriol hapten and antibody and the application thereof in a fast sensor method for uncojugated estriol detection. The structure of the hapten is scientifically designed, so that sensitivity and specificity of the antigen-antibody are improved; electro-deposition of a prussian blue-chitosan-nanogold composite membrane on the surface of an electrode is conducted, the estriol antigen is fixed to the surface of the membrane, a novel horse radish peroxidase labeled estriol antibody and a sample to be tested are added after an unbound site is sealed, the sample and a fixed antigen compete for a free enzyme labeled antibody, hydrogen peroxide is added after washing, a current signal is measured, a cyclic voltammetry estriol standard curve is obtained, the fast sensor method for uncojugated estriol detection is provided successfully, detection range is 0.001-100 ng/mL, and detection limit is 0.001 ng/mL. The method has the advantages of being easy to operate, high in sensitivity, quick, convenient and the like and can be well applied to ultra-sensitive and fast detection of estriol in the sample.
