4793-20-8

Usage

Uses

Used in Pharmaceutical Industry:
Ethyl 4-chloro-2-fluorobenzoate is used as a key intermediate in the synthesis of various pharmaceutical compounds. Its unique chemical structure allows for the development of new drugs with specific therapeutic properties.
Used in Agrochemical Industry:
In the agrochemical sector, Ethyl 4-chloro-2-fluorobenzoate serves as an essential intermediate for the production of pesticides and other agrochemicals. Its incorporation into these products contributes to their effectiveness in controlling pests and diseases in agriculture.
Used in Organic Synthesis:
Ethyl 4-chloro-2-fluorobenzoate is also utilized in organic synthesis for the preparation of various organic compounds. Its reactivity and functional groups make it a versatile building block for the creation of a wide range of chemical products.

InChI:InChI=1/C9H8ClFO2/c1-2-13-9(12)7-4-3-6(10)5-8(7)11/h3-5H,2H2,1H3

Upstream product

• 64-17-5 ethanol 
• 446-30-0 4-chloro-2-fluorobenzoic acid 

Downstream Products

• 443-26-5 2-fluoro-benzoic acid ethyl ester 
• 220389-15-1 4-cyano-2-fluoro-benzoic acid ethyl ester 

Relevant academic research and scientific papers

N′-((2-(6-bromo-2-oxo-2 H -chromen-3-yl)-1 H -indol-3-yl)methylene)benzohydrazide as a probable Bcl-2/Bcl-xL inhibitor with apoptotic and anti-metastatic potential

A wide number of marketed drugs and drug candidates in cancer clinical development contain halogen substituents. The aim of the present study was to synthesize a series of halogen incorporated indole-coumarin hybrid schiff bases - N′-((2-(2-oxo-2H-chromen-3-yl)-1H-indol-3-yl)methylene)benzohydrazides and to investigate their apoptotic and anti-migratory potential in human breast adenocarcinoma cells as well as to examine their Bcl-2 and Bcl-xL protein binding ability via in silico docking. Hybrid 5g with a bromine atom in position-7 of coumarin ring displayed significant dose dependent cytotoxic activity with high selectivity to MCF-7 cells in MTT assay. Cell cycle progression analysis of 5g treated cells using flow cytometer exhibited a cell cycle arrest in the S phase and accumulation of cells in the subG1 phase. The apoptotic mode of cell death induced by 5g was further confirmed by Annexin-V staining assay. The wound healing assay revealed a profound impairment in the migration of MCF-7 cells presumably due to down-regulation of Bcl-2 and Bcl-xL proteins induced by 5g as observed in immunoblotting analysis. SAR studies of these hybrid molecules based on cell viability and docking were also probed. The most active pharmacophore 5g was found to bind favourably to Bcl-2 and Bcl-xL in docking simulation analysis suggesting it to be a probable small molecule Bcl-2/Bcl-xL inhibitor and a potential lead for breast cancer chemotherapy with apoptotic and anti-metastatic properties.