485396-67-6Relevant academic research and scientific papers
Ultrasound-assisted enantioselective esterification of ibuprofen catalyzed by a flower-like nanobioreactor
An, Baiyi,Fan, Hailin,Wu, Zhuofu,Zheng, Lu,Wang, Lei,Wang, Zhi,Chen, Guang
, (2016)
A flower-like nanobioreactor was prepared for resolution of ibuprofen in organic solvents. Ultrasound irradiation has been used to improve the enzyme performance of APE1547 (a thermophilic esterase from the archaeon Aeropyrum pernix K1) in the enantioselective esterification. Under optimum reaction conditions (ultrasound power, 225 W; temperature, 45°C; water activity, 0.21), the immobilized APE1547 showed an excellent catalytic performance (enzyme activity, 13.26 μmol/h/mg; E value, 147.1). After ten repeated reaction batches, the nanobioreactor retained almost 100% of its initial enzyme activity and enantioselectivity. These results indicated that the combination of the immobilization method and ultrasound irradiation can enhance the enzyme performance dramatically.
Enantioselective esterification of ibuprofen under microwave irradiation
Yang, Zecheng,Niu, Xuedun,Fang, Xuedong,Chen, Ge,Zhang, Hong,Yue, Hong,Wang, Lei,Zhao, Dantong,Wang, Zhi
, p. 5472 - 5481 (2013/07/11)
Enantioselective esterification of ibuprofen has been successfully carried out in an organic solvent catalyzed by recombinant APE 1547 (a thermophilic esterase from the archaeon Aeropyrum pernix K1). Here we used microwave irradiation (MW) as the mode of heating to improve the enzyme performance. Under the optimum conditions, the enzyme activity of APE 1547 was 4.16 μmol/mg/h and the enantioselectivity (E value) was 52.9. Compared with conventional heating, the enzyme activity and the enantioselectivity were increased about 21.9-fold and 1.4-fold, respectively. The results also indicated that APE 1547 can maintain 95% of its activity even after being used five times, suggesting that the enzyme is stable under low power MW conditions.
