497919-80-9Relevant academic research and scientific papers
Glycosyl azides - An alternative way to disaccharides
Bojarova, Pavla,Petraskova, Lucie,Ferrandi, Erica Elisa,Monti, Daniela,Pelantova, Helena,Kuzma, Marek,Simerska, Pavla,Kren, Vladimir
, p. 1514 - 1520 (2007)
Glycosyl azides are shown to be efficient donors for β-galactosidases, β-glucosidases and α mannosidases. Only α-galactosidases do not cleave the respective glycosyl azide 1 and, moreover, they exhibit competitive inhibition (especially α-galactosidase fr
On expanding the repertoire of glycosynthases: Mutant β-galactosidases forming β-(1,6)-linkages
Jakeman, David L.,Withers, Stephen G.
, p. 866 - 870 (2007/10/03)
Oligosaccharide synthesis by enzymatic processes offers the potential for thrusting oligosaccharides to the forefront of pharmaceutical research, in part, due to expedient and scalable reaction protocols. Glycosynthases are an emerging class of mutant enzymes capable of synthesizing glycosidic linkages in high yield. We report a new glycosynthase enzyme generated by a point mutation of E. coli β-galactosidase that condenses α-galactosyl fluoride with aryl glucosides forming a β-(1,6) glycosidic linkage. A further point mutation within the enzyme, proximal to the active site, increases the yields significantly.
Galactosyl transfer onto p-nitrophenyl beta-D-glucoside using beta-D-galactosidase from Bacillus circulans.
Murata,Akimoto,Horimoto,Usui
, p. 1118 - 1120 (2007/10/03)
beta-D-Gal-(1-->4)-beta-D-Glc-OC6H4NO2-p and its isomers (beta-D-Gal-(1-->3)-beta-D-Glc-OC6H4NO2-p and beta-D-Gal-(1-->6)-beta-D-Glc-OC6H4NO2-p) were synthesized from lactose and beta-D-Glc-OC6H4NO2-p, using transglycosylation by the beta-D-galactosidase from Bacillus circulans. This reaction was efficient enough for us to do a one-pot preparation of galactosyl-glucoside from lactose. The order of the production of the transfer products was (1-->4) > > (1-->3) > (1-->6) in the initial stage of the reaction, and the same relationship was observed for the hydrolytic rate toward the three galactosyl-glucosides. The production of (1-->4)- and (1-->3)-linkages greatly decreased during the subsequent reaction and much more of the (1-->6)- than of the (1-->4)- and (1-->3)-transfer products was found in the later stage of the reaction.
